摘要
1980年从安徽舒城县晓天茶园的茶蚕罹病虫体中分离获得一种颗粒体病毒,经鉴定为茶蚕颗粒体病毒（AbGy）,为我国首次发现。对茶蚕3龄幼虫的半致死浓度LC50为1.23×107mg/ml,半数死亡时间LT50为5.4～8天。经感染试验,以1龄幼虫最敏感,感病死亡率随病毒浓度增大而相应提高。在感染12种昆虫中,只感染茶蚕,对桑蚕及天敌昆虫安全无伤害作用。AbGV制剂经田间防治试验示范结果,防治适期以1～2龄为宜,每亩喷施AbGV有效剂量为100～200mg,防治效果可达75.5％～97.45％,若与微量化学农药混用则有明显增效作用,病毒后效作用长达2～3年,自然感病死亡率18.76％～55.94％,AbGV的防治效果显著。
In 1980, a granulosis virus was isolated from dead larvae of Andraca bipunctata Walker in tea gardens of Shucheng County, Anhui Province, and was identified as Andraca bipunctata Granulosis Virus (AbGV). This was the first discovery of this virus in China. The LC50 of AbGV to the third instar larvae of A. bipunctata was 1.23×10-7 mg/ml, and LT50 ranged from 5.4 to 8 days. Results of the laboratory infection tests showed that the first instar larvae were the most susceptible to the virus. Larval mortality decreased with the increase of the larval instars and concentration of the virus. In the host range tests, AbGV was found to infect only A. bipunctata among the 12 insect species tested. It was safe to the silkworm, Bombyx mori and other beneficial insects. Spraying preparations of the virus at a dosage of 1,500-3,000 mg per ha. at the 1st and 2nd larval instar in field trials resulted in 75.5-97.4% control effect of A. bipunctata. Application of AbGV in conjunction with low dose of chemical insecticides considerably increased the control effect. The residual effect of AbGV in the tea gardens lasted 2-3 years. Mortality of naturally infected A. bipunctata by AbGV reached 18.7-55.9%.
关键词
茶蚕
颗粒体病毒
茶树
田间防治
4ndraca bipunctata granulosis virus tea pest microbial control
