摘要
目的 研究人参皂甙 Rb1 对脑缺血损的保护作用及其机制。方法 实验利用离体海马脑片模型 ,结合电生理学技术进行研究 :(1)观察大鼠海马脑片在缺血条件下顺向群峰电位 (orthodrom ic population spike,OPS)的变化及人参皂甙 Rb1 对它的影响。 (2 )观察谷氨酸对海马脑片 OPS的影响及人参皂甙 Rb1 抗谷氨酸毒性作用。结果 (1)使用人参皂甙 Rb1 (6 0 0 μm ol/ L)后 ,可使缺氧损伤电位 (hypoxic injury potential,HIP)出现率明显减少 ,OPS恢复率和 OPS恢复程度与对照组比较有显著性差异。 (2 )使用人参皂甙 Rb1 (6 0 0 μm ol/ L)后 ,OPS恢复率和OPS恢复程度与对照组比较有显著性差异。结论 人参皂甙 Rb1 有明显的抗脑缺血损伤作用 ,其作用机制与抗谷氨酸的神经毒性作用有关。
Objective Using rat hippocampal slices,the protective effect of ginsenoside Rb 1 on ischemic brain injury and the mechanism were investigated in vitro by means of electrophysiological technique. Methods (1)The changes of orthodromic population spike(OPS) during simulated ischemia and after administration of ginsenoside Rb 1 were observed. (2)The changes of OPS after exposure to Glutamate and the effect of ginsenoside Rb 1 aganist the Glutamate excitory toxicity were observed. Results (1)The changes of OPS after administration of ginsenosides:in control group,hypoxic injury potential(HIP) appeared in all slices during simulated ischemia,after reoxygenation the OPS recovered a little;ginsenoside Rb 1(600μmol/L) decreased the appearance of HIP,after reoxygenation,the recovery rate and amplitudes of OPS were significantly higher than those of the control group. (2)The effect of ginsenoside Rb 1 against the Glutamate excitory toxicity:in control group,Glutamate(1.5mmol/L) decreased the amplitude of OPS,after the end of Glutamate exposure,the recovery rate and amplitude of OPS were low;after administration of ginsenoside Rb 1(600μmol/L),the recovery rate and amplitude of OPS were significantly higher than those of the control group. Conclusion Ginsenoside Rb 1 has the protective effects against ischemic brain injury. The mechanism is related to the antagonist effect of ginsenosides on Glutamate excitory toxicity.
出处
《中风与神经疾病杂志》
CAS
CSCD
北大核心
2003年第5期415-417,共3页
Journal of Apoplexy and Nervous Diseases
关键词
人参皂甙RB1
大鼠
海马脑片
保护作用
顺向群峰电位
脑缺血
Ginsenoside
Hippocampal slice
Ischemia
Glutamate
Orthodromic population spike
Hypoxic injury potential
