摘要
目的:研究一种适于人成纤维细胞生长的组织工程支架材料,确定碱性成纤维细胞生长因子(bFGF)的用量。材料与方法:采用胶原与壳聚糖整合bFGF做为人成纤维细胞生长的支架材料,用体外细胞培养法确定胶原与壳聚糖在材料中的配比,用体外细胞相容性直观法观察材料与人成纤维细胞的相容性。结果:选择出了胶原与壳聚糖在材料中较佳的配比,确定了bFGF在材料中的用量范围,人成纤维细胞在胶原-壳聚糖/bFGF膜持续生长繁殖,具有生物降解性。材料与细胞呈低毒性反应。结论:胶原-壳聚糖/bFGF可做为组织工程的支架材料。
The effect of collagen-chitosan membrane with different proportionate collagen and bFGF were investigated for culture human fibroblast. The optimum weight ratio of collagen/chitoson and bFGF were selected. Using culture human fibroblast technologies and cytotoxicity evaluated in vitro- Cell morphology was observed. Experimental results show that collagen-chitosan with bFGF promoted human fibroblast adhesion and supported cell proliferation for a long time. Furthermore collagen-chitosan membrane obviously degrade after 18 d when human fibroblast was exhibited fusion spreading- compacting and stabilize. Cytotoxic to human fibroblast was revealed very low. Collagen- chitosan with bFGF should be useful as a tissue engineering biomaterial scaffold for cell culture.
出处
《中国医学物理学杂志》
CSCD
2003年第4期301-303,共3页
Chinese Journal of Medical Physics
