摘要
目的 了解中国部分地区静脉吸毒人群和不洁献血人群中艾滋病病毒 1(HIV 1)与丙型肝炎病毒 (HCV )亚型分布的相关性及其流行模式。方法 以聚合酶链反应 (PCR )技术扩增HIV 1gag的p17区和env的C2V3区 ,HCV5’NCR区和E1 E2区 ,并对扩增产物进行测序。采用ClustalW软件对所得序列进行基因树分析。结果 共检测了 2 3 9例HIV 1感染者 ,其中HCV阳性率为56.9% (13 6 2 3 9)。在 13 6例HIV 1 HCV混合感染者中 ,96.3 %是通过静脉吸毒 (IVDU )和不洁献血途径而感染的。IVDU(感染者主要来源于新疆、云南和广西 )的HIV 1亚型主要为C型 ,其HCV基因型为 1b、3a、3b和 4型。而不洁 (输 )献血人群的HIV 1主要为B型 ,其HCV基因型以 1b和 2a为主。结论 中国发现多种HIV 1亚型和HCV基因型 ,提示这两种病毒的流行是多种途径感染的结果 ,序列同源性程度之高提示这两种病毒感染是在相当近的一段时间内爆发感染的。不洁献血者HIV 1和HCV病毒株不同于IVDU的病毒株 。
ObjectiveTo determine the epidemiologic f eatures and distribution of human immunodeficiency virus-1(HIV-1) and hepatiti s C virus(HCV) infection among intravenous drug users and illegal blood donors i n China. MethodsPolymerase chain reaction (PCR) amplification and DNA sequencing were used to evaluate the HIV-1 gag p17 and env C2-V3 regions, as well as the HCV 5'NCR and E1/E2 regions. ResultsAmong 239 subjects with re ported HIV-1 infection, 56.9 % (136/239) were seropositive for anti-HCV. Of those, 96.3 % (131/136) were co-infected with HCV through intravenous drug use and illegal blood donation. Intravenous drug users in Yunnan, Guangxi and Xinjiang provinces were infected with HIV-1 subtype C and HCV genotypes 1b, 3a , 3b and 4, whereas illegal blood donors in Henan province harbored HIV-1 subt ype B' and HCV genotypes 1b and 2a. Five different HIV-1 subtypes were identi fied among 17 HIV-1-infected individuals from Beijing. ConclusionsMultiple HIV-1 subtypes and HCV genotypes were identified in China which were associated with several different modes of transmission. Homogeneity within the sequences of the two viruses suggeste d the recent, but separate, outbreaks of HIV-1 and HCV infection. The distinc t distribution patterns of HIV-1 and HCV genotypes in two high-risk groups seemed to be more closely linked to the mode of transmission than to geographic proximity.
出处
《中华流行病学杂志》
CAS
CSCD
北大核心
2003年第11期962-965,共4页
Chinese Journal of Epidemiology
