摘要
目的:研究CD14基因启动区C(-269)T分子变异与中国人群冠心病的关联。方法:用PCR技术检测了50例经冠脉造影证实的冠心病患者及90例健康人CD14基因启动区含(-269)多态位点的基因片段,HaeⅢ酶切分型。计算基因型及等位基因频率,并比较两组间差异。结果:冠心病组T等位基因(无酶切位点)频率显著高于对照组(51.00％Vs32.78％;OR=2.13;95％ CI,1.30～3.50;P<0.01)。结论:CD14基因启动区C(-269)T分子变异与中国人群冠心病相关。
Objective:To examine whether C(-269)T molecular variation in the promoter of the CD14 receptor gene is associated with coronary heart disease(CHD) in Chinese. Methods :DNA samples from 50 cases of CHD patients whose coronary lesions were confirmed by angiography, and 90 control subjects with no history of CAD and a normal resting electrocardiogram. Fragments in the promoter of CD14 receptor gene containing codon-260 polymorphic locus were amplified by PCR. and then digested with HaeIII endonuclease. Genotypes and alleles frequencies were calculated and compared between two groups. Results: A higher frequency of the T allele (absence of the cutting site) was found in CHD than in controls (51. 00% Vs32. 78% ; OR = 2. 13; 95% CI, 1. 30 to 3. 50; P<0. 01). Conclusion:In addition to the well-established risk factors, a genetically determined reaction of monocytes/macrophages to infectious stimuli could play an important role in the process of atherosclerosis.
出处
《中国误诊学杂志》
CAS
2003年第10期1458-1459,共2页
Chinese Journal of Misdiagnostics
基金
江苏省自然科学基金(BK99160)
