摘要
[目的]比较北京和太原细颗粒物PM2.5 污染水平及研究其炎性损伤毒性。[方法]选取我国具有典型污染特征的两大城市太原与北京 ,采用分级采样器和重量法收集两城市空气中细颗粒物样品 ,用甲醇超声提取细颗粒物上的B(a)P ,硝酸和过氧化氢溶解Pb ,从质量浓度、B(a)P、Pb含量等方面比较分析了两城市细颗粒物污染水平 ;同时用ELISA及RT_PCR法 ,测定细颗粒物对人肺泡上皮细胞 (A549)产生的炎性因子IL_6、TNF_α表达的影响。[结果]以美国EPA大气环境质量PM2.5 标准为参考 ,PM2.5 太原冬季、北京冬季超标率、超标倍数分别为100 % ,4.23 ;90.6 % ,2.62。太原冬季、北京冬季空气中B(a)P浓度分别为5.86 ,1.09( μg/100m3) ,均超过我国标准。总之 ,太原细颗粒物污染高于北京。细颗粒物能引起人肺上皮细胞产生炎性因子IL_6、TNF_α及其mRNA的表达增加 ,而且呈现剂量_效应关系。[结论]上述两城市的细颗粒物污染严重 。
To investigate and compare the PM 2.5 pollution levels in Taiyuan and Beijing and to study inflammation damage induced byPM 2.5 .Two cities,Taiyuan and Beijing,were selected toreflecttwodifferentairpollutiontypes.Classificaˉtion air sampler and weighting method were used for PM 2.5 collection in the air of the two cities.B(a)P in PM 2.5 was extracted by methyl alcohol using ultrasonic method and Pb in PM 2.5 dissolved in nitric acid and hydrogen peroxide.The levels of PM 2.5 pollution in mass conˉcentration and B(a)P and Pb contents were compared.Human alveolar epithelial cell line(A549)was used as target cells incubated withPM 2.5 atdifferentdoses.As inflammation cytokines,IL_6,TNF_αconcentrations were measured byanELISAkitand the mRNAexpresˉsion level of IL_6,TNF_αwere evaluated by reverse transcription polymerase chain reaction(RT_PCR).According to the Air Quality Standard for PM 2.5 of U.S.EPA,the results showed that the rate and multiple exceeding standard of PM 2.5 concentration in Taiyuan winter,Beijing winter and Beijing spring were100%,4.23;90.6%,2.62and97.4%,2.53,respectively.B(a)P in Taiyuan winterand Beijingwinterwere5.86and1.09(μg/100m 3 ),respectively,theywere overthe standard.In a word,the level ofPM 2.5 is highˉer in Taiyuan than that in Beijing.PM 2.5 exposure could induce the increase in concentration and mRNA expression of IL_6,TNF_αcomˉpared with untreated cells with a dose_effect relationship.[Conclusion]This study showed that PM 2.5 air pollution is very heavy in the two cities and PM 2.5 has potential inflammation damage.
出处
《环境与职业医学》
CAS
北大核心
2003年第5期325-330,共6页
Journal of Environmental and Occupational Medicine
基金
国家自然科学基金资助项目 (编号 :20077033)
