期刊文献+
任意字段
题名或关键词
题名
关键词
文摘
作者
第一作者
机构
刊名
分类号
参考文献
作者简介
基金资助
栏目信息

白血病细胞WT1基因启动子区DNA甲基化研究 被引量：2

Study on DNA methylation status of WT1 gene promoter in leukemia cell
原文传递
导出
摘要 目的　研究白血病细胞WT1启动子区DNA甲基化与其表达的关系。方法　采用RT PCR技术、硫化PCR结合限制性内切酶技术检测白血病细胞系及正常人外周血单个核细胞WT1基因的表达及其启动子区DNA甲基化水平。结果　正常人外周血单个核细胞及U937细胞不表达WT1基因 ,而HL 6 0、K5 6 2和KG1细胞高表达WT1基因 ,HL 6 0细胞WT1基因无甲基化。结论　WT1基因启动子区DNA甲基化不能抑制其表达 ,尚存在其他调节WT1基因表达的因素。 Objective To analyse the WT1 expression and its DNA methylation status of its promoter domain. Method The expression of WT1 gene and its DNA methylation status were assayed in leukemia cell lines and normal peripheral blood mononuclear cells (PBMNC) by RT-PCR and MS-PCR. Results WT1 was overexpressed in HL60, K562 and KG1 leukemia cell lines, but not in U937 and PBMNC. Methylation of WT1 promoter was not observed in HL60 cells. Conclusion DNA methylation of WT1 gene promotor did not inhibit its expression. Other mechanisms may appear to regulate the WT1 expression.
作者 王全顺 于力 赵瑜 韩为东 高春纪 楼方定
机构地区 解放军总医院血液科
出处 《中华血液学杂志》 CAS CSCD 北大核心 2003年第10期527-529,共3页 Chinese Journal of Hematology
关键词 白血病 WT1基因 DNA甲基化 研究 RT-PCR技术 Gene,WT1 Gene expression DNA methylation Leukemia
分类号 R733.7 [医药卫生—肿瘤]
  • 相关文献

参考文献9

  • 1Loeb DM, Sukumar S. The role of WT1 in oncogenesis: tumor suppressor or oncogene?Int J Hematol, 2002, 76:117-126.
  • 2Algar E. A Review of the Wilms' tumor 1 gene (WTI) and its role in hematopoiesis and leukemia.J Hematother Stem Cell Res, 2002, 11:589-599.
  • 3Reddy JC, Licht JD. The WTI Wilms' tumor suppressor gene:how much do we really know? Biochim Biophys Acta, 1996,1287: 1-2.
  • 4Svedberg H, Chylicki K, Baldetorp B, et al. Constitutive expression of the Wilms' tumor gene(WT1)in the leukemic cell line U937 blocks parts of the differentiation program .Oncogene, 1998, 16: 925-932.
  • 5James GH, Jeremy RG, Sanna M, et al. Mehtylation-specific PCR assay for methylation status of CpG islands. Proc Nail Acad Sci U S A, 1996,93: 9821-9826.
  • 6Desiderato L, Davey MW, Piper AA. Demethylation of the human MDR1 5' region accompanies activation of P-glycoprotein expression in HL60 multidrug resistant subline.Somat Cell Mol Gene,1997, 23:391-400.
  • 7Issa JP, Baylin SB, Herman JG. DNA methylation changes in hematologic malignancies: biologic and clinical implications. Leukemia,1997,Suppl 1: 7-11.
  • 8Issa JP, Baylin SB, Herman JG, et al. Histone deacetylase 1 inactivation by an adenovirus early gene product.Curr Biol , 2002, 12:594-598.
  • 9Gollnick P, Babitzke P. Transcription attenuation. Biochim Biophys Acta, 2002, 1577: 240-250.

同被引文献24

  • 1赵晔,陈子兴,胡绍燕,岑建农,顾伟英.造血系统肿瘤WT1基因启动子区域DNA甲基化及其调控的研究[J].中华血液学杂志,2005,26(9):517-520. 被引量：3
  • 2Barra JL, Holmes AM, Gregoire A, et al. Novel relationships among DNA methylation, histone modifications and gene expression in Ascobolus[J]. Mol Microbiol, 2005,57(1):180.
  • 3David ML, Ella E, Chirag BP, et al. Wilms'tumor suppressor gene(WT1) is expressed in primary breast tumors despite tumor specific promoter methylation [J]. Cancer Res,2001,61:921.
  • 4Beillard E, Pallisgaard N, van der Velden VHJ, et al. Evaluation of candidate control genes for diagnosis and residual disease detectionin leukemic patients using realtime quantitative reverse transcriptase polymerase chain reaction(FQPCR)-a Europe against cancer program[J].Leukemia, 2003,17 (12) : 2474.
  • 5Ogawa H,Tamaki H, Ikegame K, et al. The usefulness of monitoring WT1 gene transcripts for the prediction and management of relapse following allogeneic stem cell transplantation in acute type leukemia [J].Blood, 2003, 101:1698.
  • 6Messing A, Brenner M. GFAP: Functional implications gleaned from studies of genetically engineered mice[J]. Glia,2003,43(1) :87.
  • 7Kaneuchi M, Sasaki M, Tanaka Y, et al. WT1 and WT1-AS genes are inactivated by promoter methylation in ovarian clear celt adenocarcinoma[J]. Cancer, 2005,104 ( 9 ) : 1924.
  • 8Merl A, Herman JG, Mao L, et al. 5'-CpG island methylation is associated with transcriptional silencing of the tumor suppressor P16/CDKN2/MTS in human cancers. Nat Med, 1995,1: 686-692.
  • 9Cody DT, Huang Y, Dargy CJ, et al. Differential DNA methylation of the p16 INK4A/CDKN2A promoter in human oral cancer cells and normal human oral kerarinocytes. Oral Oncol, 1999, 35: 516-522.
  • 10James GH, Jeremy RG, Sanna M, et al. Methylation-specific PCR:a novel PCR for methylation status of CpG islands. Proc Natl Acad Sci U S A, 1996, 93: 9821-9826.

引证文献2

二级引证文献3

中华血液学杂志
2003年 第10期

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部