摘要
目的 观察人芽囊原虫包囊在不同环境中的光学和超微结构的形态特点。 方法 从粪便分离人芽囊原虫 ,转种至改良的Jones培养基培养 72h后 ,再转种至混合纤维素脂微孔滤膜成囊培养基和Suresh成囊培养基中培养。采用光学和电子显微镜对培养基中与粪便中的包囊进行光学和超微结构的观察。 结果 光镜下观察 ,新鲜粪便中的包囊微小圆球形 ,具有折光性 ,不同的个体大小悬殊 ,HE染色可显示囊壁有 3层。用Suresh成囊培养基培养的包囊圆形或椭圆形 ,中心体色淡 ,内有大小不等的孢子体。混合纤维素脂微孔滤膜培养的包囊均较大 ,有空泡型、颗粒型、薄壁与厚壁包囊之分。透射电镜下观察 ,无纤维层的裸露包囊形态大都是圆形或卵圆形。包囊直径为 14 μm。包囊呈典型的人芽囊原虫核 ,其线粒体较多 ,胞质中糖原呈大小不一块状。 结论 包囊在不同环境中的形态基本相同、大小不一、结构多型。
Objective To observe the light microscope appearance and ultra-structure of Blastocystis hominis cysts in different environment. Methods B. hominis were detached from human feces, and then turned to improved Jones medium culture for 72 hours, then turned into synthetic fabric element grease tiny hole dialyzer cyst culture medium and Suresh cyst medium culture. The cysts in culture medium and human feces were observed by light and electric microscope. Results Under the light microscope, the cysts in feces were refractive tiny pellet, and their sizes was great disparity, HE pigmentation showed that cyst wall had three layers; the cysts in Suresh cyst medium culture were round or ellipse, the color of the centrosome was weak, and there were different sizes of sporophytes; the cysts in synthetic fabric element grease tiny bore dialyzer culture were bigger with vacuolized type, granular type, thin and thick type. Under transmission electric microscope, many naked cysts without the fibrin layer were circular or oval in shape, and 14 μm in diameter, the nuclear structure presented typical B. hominis and multiple mitochondrian; glycogen in the cytoplasm was presented clump of different size. Conclusion The B. hominis cysts in different environment were basic sameness in shape, different in size and multiple types in structure. (For Fig. 1-10, to see back cover)
出处
《中国寄生虫病防治杂志》
CSCD
2003年第5期287-289,F004,共4页
Chinese Journal of Parasitic Disease Control
基金
江西省自然科学基金资助课题 (No .0 1 4 0 0 2 0 )
关键词
人芽囊原虫包囊
光学形态
超微结构
Blastocystis hominis cysts
light microscope appearance
ultrastructure
