摘要
对 1 996年至 2 0 0 1年间自我国部分养鸡场发病鸡或死亡鸡分离鉴定的 8株H9N2亚型禽流感病毒的非结构蛋白基因 (NS1 )进行了扩增和序列测定 ,并分析和比较了其核苷酸和氨基酸的同源性。结果表明 ,NS1基因核苷酸和氨基酸同源性分别为 96 5 %～ 99 5 %和94 5～ 98 6% ,说明NS1基因在遗传进化上高度保守 ,稳定遗传。与中国香港、韩国、巴基斯坦及人源H9N2分离株相比较 ,发现中国大陆的鸡源H9N2分离株的NS1基因在其羧基端缺少 1 3个氨基酸。系统进化树分析表明 ,该 8株病毒的NS1基因属于相同的进化分支 ,而且中国的早年分离株A chicken Beijing 1 94位于该进化分支的根部 ,暗示这些分离株的NS1基因是由A chicken Beijing 1 94演化而来 ;尚未发现NS1基因属于A quail HongKong G1 97 like分支的分离株。同时 ,系统进化树也说明了我国的H9N2分离株与韩国、巴基斯坦等地的H9N2分离株隶属于不同的进化分支 。
The nonstructural protein(NS1) genes of eight H9N2 subtype influenza virus strai ns isolated from diseased chickens on different farms during 1996～2001 were amp lified and sequenced. The nucleotide and deduced amino acid sequences of NS1 gen es of these isolates were compared. The results showed that the homologies of nu cleotide and amino acid of the isolates were 96 5%～99 5% and 94 5～98 6%, r espectively. These indicated that NS1 genes of H9N2 influenza viruses isolated i n China were well conserved. Comparison of the amino acid sequences of NS1 genes of these isolates with those of H9N2 viruses isolated in Hong Kong of China, Ko rea and Pakistan demonstrated that nonstructural(NS1) proteins of the eight stra ins had a deletion of 13 amino acid residues at the carboxy terminus. Phylogenet ic analyses showed NS1 genes of these isolates belonged to the same lineage and derived from an early chicken H9 virus isolated in 1994 The NS1 gen es of the eight isolates belong to different sublineages from those of the H9N2 viruses isolated in Hong Kong, Korea and Pakistan, suggesting that the geographi cal distribution plays a significant role in the evolution of the H9N2 subtype i nfluenza viruses.
出处
《微生物学报》
CAS
CSCD
北大核心
2003年第5期547-553,共7页
Acta Microbiologica Sinica
