摘要
目的 :探讨氟对体外培养破骨细胞的影响。方法 :从新生兔四肢长骨中分离出破骨细胞 ,将其与盖玻片、骨片共同培养 ,于培养 6 h后加入不同浓度 (10 - 7～ 10 - 3mol/L)的氟化钠 (Na F) ,用酶组织化学染色法测定 Na F对破骨细胞的作用 ,用倒置相差显微镜和图像分析处理系统对骨吸收陷窝的数目和面积进行检测。结果 :Na F使抗酒石酸酸性磷酸酶(TRAP)染色阳性的多核细胞 ,即破骨细胞数目减少。染氟 10 - 5m ol/L、 10 - 4mol/L、 10 - 3mol/L 时 ,破骨细胞数目与对照组相比差异有显著性 (P<0 .0 5 ) ,细胞体积缩小 ,胞浆空泡增多 ,其中 10 - 3m ol/L Na F组破骨细胞改变尤为明显。与对照组相比 ,10 - 5mol/L、 10 - 4mol/L、 10 - 3mol/L Na F组骨片上骨吸收陷窝数量和面积均减少 (P<0 .0 5 ) ,而且陷窝周缘也变得模糊。结论 :在本实验浓度和时间范围内 ,Na F可直接损伤破骨细胞并抑制破骨细胞的骨吸收功能。
Objective:To study the influence of sodium fluoride(NaF)on osteoclast in vitro.Methods:Osteoclast isolated from the long bone of neonatal rabbits were cultured on glass and bone slices.After cultured 6 hours,different concentrations(10 -7 mol/L～10 -3 mol/L)of NaF were administered.The effect of NaF on osteoclast was examined by the method of enzyme histochemistry staining.The number and area of lacuna formed by osteoclast resorption on bone slices were observed and analyzed with phase-contract microscope and image analysis apparatus.Results:NaF (10 -5 mol/L,10 -4 mol/L,10 -3 mol/L) made osteoclast which was identified as tartrate resistant acid phosphatase (TRAP) staining positive multinucleated cell on glass slices count reduced,volume decreased and vacuolar developed,especially at the concentration of 10 -3 mol/L( P <0 05).In addition,compared with the control group,the number and area of bone resorption lacuna on bone slices reduced in the group given 10 -5 mol/L,10 -4 mol/L,10 -3 mol/L NaF ( P <0 05).Furthermore,the border of resorption lacuna became clouding.Conclusion:Within experimental concentrations and time,NaF could directly destroy osteoclast and inhibit bone resorption function of osteoclast in vitro.
出处
《现代预防医学》
CAS
2003年第5期617-619,共3页
Modern Preventive Medicine
基金
国家自然科学基金 (编号 :3 0 160 0 79
名称:氟对骨骼损伤机制的分子生物学研究)
