摘要
作者对小鼠肝癌肿瘤浸润性淋巴细胞(Tumor-infiltratjng lymphocyte,TIL)的分离和培养进行了初步研究。结果表明:运用酶消化加Ficoll或Percoll不连续密度离心法均能有效地获得TIL,两者相比,后者分得的TIL纯度更高(P<0.01)。TIL在含2000u/ml重组白细胞介素2(recombinant IL-2,rIL-2)RPMI 1640培养基中被激活,5～7 d后开始扩增,呈集落样生长,同时肿瘤细胞逐渐减少,至14d左右基本消失。培养40d时TIL扩增到原来的10~5倍,致分裂素PHA刺激的脾细胞上清液或LAK细胞上清液(称条件培养基)可促进TIL的扩增。
By using enzyme digestion and Fiooll or Percoll discontinuous density methods, we have successfully obtained tumor-infiltrating lymphocytes (TIL) from mouse hepatoma. When analyzing the purity of TIL after separation, we found that Percoll is more effective than Ficoll (P<0.01). TIL could be activated in the presence of rIL-2 and began to expand after culturing for 5-7 days, the tumor cells tend to decrease and disappeared at day 14 or so. TIL increased 105-fold over 40 days. Conditioned medium containing supernatant of PHA and rIL-2 stimulated syngeneic spleen cell culture could promote the expansion of TIL.
出处
《上海医科大学学报》
CSCD
1992年第3期201-205,共5页
Journal of Fudan University(Medical Science)
基金
国家教委高教博士点(1292107)
国家自然科学基金(39170815)
关键词
肝肿瘤
TIL
分离
培养
小鼠
mouse hepatoma
tumor infiltraing lymphocyte
Ficoll
Pereoll
conditioned medium
