摘要
筛选水稻体细胞无性系,是品种改良的有效途径之一,而继代培养可望获得较大幅度的性状变异。本研究以三个水稻品种的幼穗为外植体,分析了不同继代再生植株的性状变异动态,结果表明,继代培养对不同品种绿苗分化率有显著影响,随着继代培养代数的增加,绿苗分化率接近倒“V”形分布,不同继代绿苗分化率及再生植株农艺性状在品种间差异显著,不同继代的再生植株出现了育性、芒形及粒形等性状变异。
Selection of rice somaclonal variants is one of effective approaches to the varietal improvement. A wide range of somaclonal variations in agronomic characters were obtained through subcultures: Ther immature panicles of cultivar Hua Han Zao, Tie Gui Feng, and 8411 were used as the initial explants of in vitro culture. The characteristical variations of the regenerated plants from various subcultures were analysed. The results showed that the plant regeneration rates of different varieties were affected by subcultures. The regeneration rates in different subcultures appeared to be distributed as a reversed V-type in 3 rice cultivars. The plant regeneration rates and the 6 agronomic characters of the regenrated plants from subcultures showed a significant difference among 3 cultivars. In addition, other characteristic variations such as in sterility, awaedness and grain size were found among somaclones.
出处
《上海农业学报》
CSCD
1992年第1期25-30,共6页
Acta Agriculturae Shanghai
关键词
水稻
继代培养
再生植株
农艺性状
Rice, Immature panicle culture, Subeulture, Regenerated plants, Agronomic characters, ANOVA
