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米曲霉中提取的β-D-呋喃果糖苷酶的浓缩与纯化 被引量:1

Concentration and Purification of β-D-Fructofuranosidase EC3. 4. 1. 26)from Aspergillus.oryzae
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摘要 采用热纯化、乙醇沉淀法、超滤法和盐析法四种方法对从米曲霉中提取的β- D-呋喃果糖苷酶进行浓缩与纯化。试验结果表明热处理对β- D-呋喃果糖苷酶的纯化效果不明显;乙醇法浓缩粗酶液,可以得到浓缩的酶液 (2. 5倍 ),活力明显提高;盐析法对β- D-呋喃果糖苷酶活性损失较大;超滤法浓缩酶液时间长,浓缩倍数低,实际应用意义不大。 β- D- fructofuranosidase is extracted from an Aspergillus.oryzae(EC3. 4. 1. 26).The four methods of heating、 alcohol deposition、 ultralfiltration and salting out are used to concentration and purification ofβ- D- fructofuranosidase.Alcohol deposition is a desirable method to concentrantion ofβ- D- fructofuranosidase.
作者 马莺 骆承庠
出处 《食品科技》 CAS 北大核心 2001年第1期47-48,55,共3页 Food Science and Technology
关键词 β—D—呋喃果糖苷酶 米曲霉 超滤法 β-D-fructofuranosidase Aspergillus oryzaes ultralfiltration
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  • 1ParkYK,AlmeidaMM.ProductionoffructooligosaccharidesfromsucrosebyatransfructosylasefromAspergillusniger.WorldJMicrobiolBiotechnol,1991,7:331~334
  • 2VanBalken,JAM,VanDoorenThIGM,VandenTweelWJJ.Productionof1-kestosewithintactmyceliumofAspergillusphoeniciscontainingsucrose-1-fructosy1transferase.ApplMicrobiol.Biotechnol,1991,35:216~221
  • 3HayashiS,MatsuzakiK,InomataY,TakasakiY,ImadaK.PropertiesofAspergillusjaponicusβ-fructofuranosidaseimmobilisedonporoussilica.WorldJMicrobiolBiotechnol,1993,9:216~220
  • 4HSugimoto.RemovalofoligosaccharidesfromsoymilkbyanenzymefromAspergillusSatitoi.JournalofFoodScience,1970,35:655~660
  • 5VLSBaldini,ISDraetta,YongKPank.Purificationandcharacterizationofα-galactosidasefromFeijaobeanPhaseolusvulgars.JournalofFoodScience.1985,50:1766~1767
  • 6SmileyKL,Hensley,DE,GasdorfHJ.Alpha-galactosidaseproductionanduseinahollow-fiberreactor.ApplEnvironMicrobiol,1976,31:615~617
  • 7MugheeJE,SilmanRBagleyEB.ProductionofAlpha-galactosidasefromAspergillusawamori:propertiesandactiononpara-nitrophenyl-α-D-galactopyranosideandgalacto-oligosaccharidesofsoymilk.J.Am.OilChem.1978,153:375~380
  • 8ThananunkuiD,TanakaM,ChichesterCO,LeeTC.DegradationofraffinoseandstachyoseinsoybeanmilkbyAlpha-galactosidasefromMorterellavinacea.JFoodSci,1976,41:173~175
  • 9RCruz,TCBatistela,GWosiacki.Microbialα-galactosidaseforsoymilkprocessing.JourmalofFoodScience,1981,46:1196~1200
  • 10HiroyukiHASHOIMOTO,ChieKATAYAMA,MasaruGOTO,TatsuyukiOKINAGA,SumioKITAHATA.Enzymaticsynthesisofα-linkedgalactooligosaccharidesusingthereversereactionofacell-boundα-galactosidasefromCandidaguilliermondiiH-404.BiosciBiotechBiochem,1995,59(2):179~183

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  • 2单黎然,龚月桦,贾建光,罗瑛婕.4种重要功能性低聚糖的研究进展[J].西北农林科技大学学报(自然科学版),2006,34(7):96-100. 被引量:24
  • 3PLAYNE M J, CRITYENDEN R G. Galacto-oligosaccharides and other products derived from Lactose [ J ]. Adv Dairy Chem,2009,3 ( 期号) : 121-201.
  • 4AKIHIRO T, JIRO K, SATOKO O. Production of Lactosucrose by Bacil- lus natto Levansucrase and some proper ties of the enzyme [ J]. Nippon Shokuhin Kogyo Gakkaishi, 1991,38 (9) :789-796.
  • 5YANASE H, IWATA M, NAKAHIGASHI R, et al. Purification, crys- tallization, andproperties of the extracellular levansucrase from Zy- momonas mobilis [ J ]. Biosci Biotech Biochem, 1992,56 ( 8 ) : 1335- 1337.
  • 6PARK N H, CHOI H J, OH D K. Lactosucmse production by various microorganisms harboring levansucrase activity [ J ]. Biotechnol Lett, 2005,27 ( 7 ) :495-497.
  • 7TANG L B, LENSTRA R, BORCHERT T V, et al. Isolation and char- acterization of levansucrase-encoding gene from Bacillus amyloliquefa- ciens [ J]. Gene, 1990,96( 1 ) :89-93.
  • 8HETI'WER U, GROSS M, RUDOLPH K. Purification and characteriza- tion of an extracellular levansucrase from Pseudomonas syringae pv. Phaseolicola [ J]. J Bacteriol, 1995,177 (10) :2834-2839.
  • 9OHTSUKA K, HINO S, FUKUSHIMA T, et al. Characterization of levansucrase from Rahnella aquatilis JCM-1683 [ J ]. Biosci Biotech Biochem, 1992,56 ( 9 ) : 1373 -1377.
  • 10TIEKING M, EHRMANN M A, VOGEL R F, et al. Molecular and functional characterization of a levansucrase from the sourdough isolate Lactobacillus sanfranciscensis TMW 1. 392 [ J ]. Appl Microbiol Bio- technol,2005,66(6) :655-663.

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