摘要
作者借助BALB/C小鼠柯萨奇B3m(CoxB3m)病毒性心肌炎模型,用单克隆抗体间接免疫荧光法测定病毒感染后不同天数小鼠脾脏的T细胞亚群;用LDH释放法测定NK细胞活性。结果显示:脾脏的Thy-1(Pan T)、L3T4(TH/I)及Ly-2(TS)细胞在感染后第3天起开始下降,直至第14天,各点所测得的值显著低于正常对照(P<0.05～0.001);T4/T8(L3T4/Ly-2)比值从感染后第3天的2.08±0.97上升到14天时的2.68±0.58;NK细胞活性在感染后呈进行性下降,各点所测得的值均显著低于正常对照(P<0.05～0.001)。这些实验结果提示:细胞免疫异常在本病发病中起一定作用。
By means of murine model of Coxsakie B3m Viral myocarditis. We used monoclonal antibody with immunofluorescence technique to study the serial changes in T lymphocyte subset and also used LDH release method to study the NK cell activity in spleen of the experimental mice on various days after infection. Results showed that Thy-1^+(pan T). L3T4^+(TH/I) and Ly-2^+(TS) cells in spleen were decreased from d3-d14 after virus inoculation and within these days all the Yalues were significantly less than those in the normal control group (P<0.05-0.001). The ratio of T4/T8(L3T4^+/Ly-2^+)was increased from d3(2.08±0.97) to d14(2.68±0.58)after virus infection. The NK cell activity had been decreasing after infection and all the values were significantly lower than those in the control group (P<0.00-0.001). These results revealed that abnormalities of cell-mediated immunity played importent role in the pathogenesis of this disease.
出处
《上海第二医科大学学报》
CSCD
1992年第4期304-307,共4页
Acta Universitatis Medicinalis Secondae Shanghai
关键词
心肌炎
柯萨奇病毒
T细胞
NK细胞
animal model myocarditis NK cell activity Coxsackie B_(3m) virus Tlymphocyte subset
