摘要
目的 探讨脐血造血细胞的低温保存方法。 方法 分别用程控降温液氮保存法、非程控降温液氮保存法和-80℃非程控降温保存法保存脐血有核细胞。结果 3种方法保存脐血有核细胞均获得较满意的结果。结论 用羟乙基淀粉 ( HES)自然沉淀法和低速离心法分离脐血有核细胞 ,获得较满意的回收率 ;用 -80℃非程控降温保存法保存脐血有核细胞 ,可降低二甲基亚砜 ( DMSO)对细胞的毒性作用 ,适用于短期保存造血细胞。
Objective To study the methods of cryopreservation of hematopoietic cell. Methods Three methods were used to cryopreserve the neuclear cells of cord blood which were cryopreserved in liquid nitrogen with and without rate-controlled freezing,and in -80℃ refrigerator without rate-controlled freezing. ResultsThe three methods were fit for cryopreservation of the neuclear cells of cord blood. Conclusions The recovery rate of neuclear cells of cord blood is gratifying using the isolation method of 6% hetastarch(HES) sedimentation and low speed centrifuge. It can decrease the toxicity of DMSO using the method of cryopreservation in -80℃ refrigerator without rate-controlled freezing which is fit for preservation of hematopoietic cells within short time.
出处
《临床输血与检验》
CAS
2003年第4期248-250,共3页
Journal of Clinical Transfusion and Laboratory Medicine
关键词
脐血造血干细胞
低温保存
液氮
有核细胞
细胞分离
Umbilical cord blood Hematopoietic cells Cryopreservation Reagent of cryopreservation
