摘要
目的 研究FESS围手术期鼻部粘膜AQP1表达变化与鼻腔分泌物的增多和粘膜组织水肿的关系。方法 选取Ⅰ型和Ⅱ型鼻窦炎患者各 10例 ,按FESS围手术期分成术前组、术后 1～ 2周组、术后 4～ 6周组和术后 8～ 12周组 ;空白对照组患者10例。利用免疫组织化学技术分别观察围手术期各实验组AQP1的表达分布及围手术期AQP1相对量的变化情况。结果 AQP1在各实验组中表达于粘膜粘液腺细胞的胞浆中 ,上皮层少量表达 ;AQP1在Ⅰ型和Ⅱ型鼻窦炎围手术期各实验组中阳性细胞的计数 :术前组与术后 1～ 2周组间差异无显著意义 (P >0 .0 5 ) ;术后 8～ 12周组与对照组间差异无显著意义 (P >0 .0 5 ) ;其余各组之间均有显著性差异 (P <0 .0 1)。结论 (1)AQP1表达可能与FESS围手术期患者鼻腔粘膜水肿形成和消退有关 ;(2 )AQP1的表达可能与FESS围手术期患者鼻腔分泌物的多少有关。
Objective To study the expressions of aquaporin1(AQP 1)in nasal polyp,sinusitis and perioperative period of FESS,and to study the influence of FESS on the expressions of AQP 1,so as to clarify the significance of AQP 1 inducing more nasal secretions and edema.Methods Ten cases of type I and 10 patients of type II were enrolled,at the same time,other groups also classified based on various stages during the perioperative period:preoperative group,1-2week group after FESS,4-6week group and 8-12 week group,each group was made up of 10 cases; control group was set as well which were the patients with septal deviation inspite of other diseases.Underwent immunohistochemical techniques of nasal mucosa,expressions and distributions of AQP 1 and alternations of relative quantities of perioperative AQP 1 in the mucosa of different study groups were observed and measured.Results AQP 1 expressed mainly in cytoplasm of mucous gland cells,and slimly in epithelial lining of all trial groups; AQP 1 countings in positive cells of all trial groups the difference between preoperative group and 1-2 week group after FESS didn′t have significance(P>0.05);which hadn′t significant difference between 8-12 week group after FESS and control group(P>0.05);AQP 1 countings among other groups had signficance(P<0.01) each other.Conclusions (1) AQP 1 might be a vital factor for the formation and elimination of mucosal edema in operative fields after FESS;(2) Changes of AQP 1 expression might be a participant in the alternations of nasal discharges after FESS.
出处
《重庆医学》
CAS
CSCD
2003年第11期1456-1459,共4页
Chongqing medicine
