摘要
目的 研究豚鼠耳蜗微血管内皮细胞的体外通透性特征。方法 (1)用小池技术建立起内皮细胞通透性的体外模型 ;(2 )研究该体外模型耳蜗微血管内皮细胞的跨细胞电阻及对12 5I 牛血清白蛋白的通透曲线 ,并与脑及肺的内皮细胞通透性进行对照。结果 (1) 3种内皮细胞的体外模型中加入12 5I 牛血清白蛋白后 ,贝克 时间变化图均呈抛物线型上升曲线 ,其通透性从小到大依次为脑、耳蜗、肺内皮细胞 ,各组间相差显著 (P <0 .0 1) ;(2 )耳蜗、脑及肺 3种内皮细胞在培养增殖过程中其跨细胞电阻呈动态增加过程 ,以 5× 10 4/cm2 的细胞密度接种时 ,7d左右电阻均到达峰值 ,其跨细胞电阻峰值大小分别为脑 (2 4 4 .7± 4 6 .9Ω/cm2 ) >耳蜗 (118.9± 18.5Ω/cm2 ) >肺 (4 6 .6± 5 .9Ω/cm2 ) ,且各组间相差显著 (P <0 .0 1)。结论 该模型下耳蜗微血管内皮细胞的通透性与其活体时的通透性特征基本一致。
Objective To study the permeability in vitro of cochlear microvascular endothelial cells (CMEC) from guinea pigs.Method (1)Established the model in vitro for the permeability of endothelium using small pool technique; (2)Researched the model′s transendothelial electric resistance (TEER) on CMEC and its permeability on radioiodinated 125 I bovine serum album (RISA),while contrasting with brain microvascular endothelial cells (BMEC) and pulmo microvascular endothelial cells (PMEC).Result (1)After RISA administration,the Bake-time curve of the models in vitro on the three endothelial cells took an ascending parabola,the permeability of endothelium sorted from great to small BMEC,CMEC and PMEC,and giving an obviously significant difference (P<0.01)each other;(2)The TEER increased dynamically and reached the peak about seven days after inoculation of culture cells at 5×10 4/cm 2 level during the CMEC,LMEC and BMEC proliferated,the peak values was BMEC(244.7±46.9Ω/cm 2)>CMEC(118.9±18.5Ω/cm 2)>LMEC(46.6±5.9Ω/cm 2),also giving an obviously significant difference (P<0.01)each other.Conclusion The permeability of CMEC on this model in vitro correspond with one in vivo in the main.
出处
《重庆医学》
CAS
CSCD
2003年第11期1444-1446,共3页
Chongqing medicine
基金
国家自然科学基金资助项目 (39870 834)
