摘要
目的 观察抗CD3单克隆抗体激活的杀伤 (CD3AK)细胞过继回输治疗乙型肝炎的近期治疗效果。方法 从乙肝病人外周血获得单个核细胞 (PBMCs) ,用抗CD3单抗和IL_2体外诱导培养 ,7天后将培养的CD3AK细胞回输给病人 ;平均 1个疗程回输细胞数为 8～ 10× 10 9;检测T细胞亚群和HBVDNA含量变化情况。结果 CD3AK细胞过继回输治疗后 ,CD3 + 、CD4+ 、CD8+ 细胞均比治疗前明显增高 ;随着免疫治疗时间的延长 ,HBVDNA含量呈下降趋势。结论 CD3AK细胞过继免疫治疗可增强患者的细胞免疫功能 ,降低血清病毒含量 ,是一种安全有效的乙肝治疗方法。
Objective To observe the short_term therapeutic effects of adoptive transfer of autologous anti_CD3 antibody activated killer (CD3AK) cells on patients with hepatitis B. Methods The peripheral blood mononuclear cells (PBMCs) of patients with hepatitis B were separated and induced into CD3AK cells in culture medium containing anti_CD3 an d IL_2 in vitro .After 7 days,the cultured CD3AK cells were transfused back t o these patients.The numbers of CD3AK cells transferred to the patients were 8～ 10×10 9 in one period of treatment.The changes of T lymphocyte subsets and the levels of serum HBV DNA were determined. ResultsAfter the treatment with CD3AK cells,the expressions of CD3 +,CD4 +,CD8 + molecules on PBMCs cells of the patients were higher t han their expressions before cure;Along with time prolonged,the levels of serum HBV DNA showed degressive trend. Conclusion Adoptive immunotherapy of autologous CD3AK cells can significantly enhance cell u lar immune functions of the patients with hepatitis B and reduce the levels of serum HBV DNA.It is a safe and effective treatment for the patients with hepatit is B.
出处
《胃肠病学和肝病学杂志》
CAS
2003年第5期467-469,共3页
Chinese Journal of Gastroenterology and Hepatology
