摘要
目的 探讨大鼠肠缺血再灌注肝损伤后肝组织bcl 2、Bax蛋白的表达及黄芪注射液对其表达的影响。方法 通过夹闭肠系膜上动脉建立肠缺血再灌注肝损伤模型 ,采用免疫组织化学方法与医学图像分析检测假手术组、模型组及黄芪注射液组大鼠肝组织bcl 2、Bax蛋白表达的阳性表达指数。结果 ①与假手术组比较 ,模型组肝组织bcl 2、Bax蛋白的阳性表达指数升高 (P <0 0 1)。②与模型组比较 ,黄芪注射液组肝组织bcl 2蛋白的阳性表达指数升高 (P <0 0 1) ,Bax蛋白的阳性表达指数降低 (P <0 0 1)。结论 大鼠肠缺血再灌注肝损伤后肝组织bcl 2、Bax的表达明显升高 ;黄芪注射可增强bcl 2的表达、抑制Bax的表达。这可能是黄芪防护大鼠肠缺血再灌注肝损伤的机理之一。
Objective To study the expressions of bcl-2 and Bax protein after liver injury induced by intestinal ischemia-reperfusion and the effect of Astragalus membranaceus injec tions.MethodsThe model of liver injury induced by intestinal ischemi a-reperfusion was established by blocking the superior mesenteric artery with c lap.The immunohistochemistry and MIPS were used to measure bcl-2 and Bax protei n positive expression indexes in rats hepatic sham-operated group,model grou p,Astragalus membranaceus injections group.Results①bcl-2 and Bax protein positive expression indexes in m odel group were higher than those in sham-operated group,( P <0.01).②bcl-2 p rotein positive expression index in Astragalus membranaceus injections group was higher than that in model group,( P <0.01);Bax protein positive expression i ndex in Astragalus membranaceus injections group was lower than that in model gr oup ( P <0.01);ConclusionExpressions of bcl-2 and Bax prot e in increase after liver injury induced by intestinal ischemia-reperfusion in r ats;Astragalus membranaceus injections can enhance the expression of bcl-2 protein and reduce the expression of Bax protein.It may be one of mechanism s of Astragalus membranaceus injections to protect liver injury induced by inte stinal ischemia-reperfusion in rats.
出处
《胃肠病学和肝病学杂志》
CAS
2003年第5期427-428,共2页
Chinese Journal of Gastroenterology and Hepatology
