摘要
目的 探讨血清细胞因子水平与乙型肝炎病变的关系。方法 运用ELISA法、比色法及荧光定量PCR技术检测不同类型乙型肝炎患者血清细胞因子、ALT、TBil及HBV DNA水平 ,观察患者治疗前后细胞因子变化及其与预后的关系。结果 除隐匿性肝炎外 ,急性、慢性及重型肝炎患者血清TNF α、IL 6、IL 8、IL 12、IL 18的水平均显著高于对照组(P <0 0 1) ,升高程度与病情相关 ;急性、慢性肝炎经治疗后 ,细胞因子浓度均明显下降 (P <0 0 0 5～ 0 0 0 1) ,重型肝炎患者无明显变化 (P >0 0 5 ) ;预后好的患者细胞因子水平下降更为明显 (P <0 0 5 ) ;相关分析表明 :乙肝患者各细胞因子与血清ALT、TBil水平相关 ,与HBV DNA无相关性。结论 血中细胞因子水平是判断乙肝患者免疫状态。
Objective To investigate the relation ship between serum cytokines and hepatitis B Methods The levels of serum cytokines,ALT,TBil and HBV DNA were measured in 96 patients with hepatitis B and 22 normal controls by ELISA,colorimetry and quantitative fluorescence PCR technique Results Except latent hepatitis,the level of serum TNF α,IL 6,IL 8,IL 12 and IL 18 in different type of hepatitis B were significantly higher than those of controls( P <0 001);The cytokines in patients with acute and chronic hepatitis B decreased significantly after treatment( P <0 005, P <0 001) and the patients with favourable prognosis had more decreased seram cytolines than patients with doubtful prognosis( P <0 05) Serum levels of cytokines were positively correlated with the ALT,TBil(r:0 51～0 73, P <0 05) but not correlated with HBV DNA(r: 0 028～0 049, P >0 05) Conclusion Detecting blood cytokines can reflect the state of immunity,liver injury and prognosis for hepatitis B
出处
《四川医学》
CAS
2003年第11期1111-1112,共2页
Sichuan Medical Journal
