摘要
目的:建立人血清中双嘧达莫的浓度测定方法。方法:采用高效液相色谱法,色谱柱:大连Nova-Pak C_(18)(4 μm,150mm×4.0mm);柱温:40℃;流动相为甲醇-0.1％磷酸氢二钠(75:25);流速:0.8 mL·min^(-1);检测波长:285 nm。结果:双嘧达莫血药浓度在0.483～61.76 mg·L^(-1)范围内线性良好,相关系数为0.9999,最小检测浓度为0.193 g·L^(-1);测得方法回收率及提取回收率分别为95.41％～106.75％和78.72％～89.97％,日内和日间RSD均小于7％。结论:本方法简便、准确,适合于双嘧达莫的血药浓度监测及药动学研究。
OBJECTIVE To establish an HPLC method for the determination of dipyridamole concentration in serum. METHODS Nova-Pak C18 column was used with methanol-0.1 % sodium phosphate dibasic anhydrous (75 :25) as mobile phase, the flow rate was 0.8 mL·min-1,detection wavelength was 285 nm, temperature was 40℃. RESULTS The linear rang of serum concentration was 0.483 - 61.76 mg·L -1 ( r = 0.9999), detection was 0.193 mg·L-1. The relative and extractive recoveries were 95.41 % ~ 106.75 % and 78.72 % ~ 89.97 % . The RSD for inter-day and intra-day were all less than 7% . CONCLUSIONS The method is simple and accurate, and suitable for the determination of dipyridamole in serum and its pharmacodynamics.
出处
《中国医院药学杂志》
CAS
CSCD
北大核心
2003年第11期668-669,共2页
Chinese Journal of Hospital Pharmacy
关键词
双嘧达莫
高效液相色谱法
血药浓度
dipyridamole
HPLC
drug concentration in plarmacodynamics
