摘要
通过 PCR方法克隆了胸膜肺炎放线杆菌 ( Actinobacillus pleuropneum oniae)武汉株完整的 apx C基因和部分apx A基因约 3.4 kb。将该 DNA片段克隆到载体 p Bluescript SK( + )中得到质粒 p SKPP,并进一步将 gfp基因插入到 apx C基因的 Xba1 位点处 ,构建了 apx C基因插入失活的转移载体 p SKPG,从而为构建胸膜肺炎放线杆菌apx
In the study,the 3 4 kb DNA fragment including apxⅡC gene and part of apxⅡC gene was amplified by PCR and cloned into the plasmid pBluscriptⅡ SK+,and the recombinant plasmid was denominated pSKPP.The gfp gene was inserted into Xba1Ⅰ site of pSKPP,and the Xba1Ⅰ site locats in the apxⅡC gene.So the transfer vector pSKPG was constructed.This work will lay a basis for further research on candidate vaccine strain against Actinobacillus pleuropneumoniae.
出处
《中国兽医学报》
CAS
CSCD
北大核心
2003年第6期551-554,共4页
Chinese Journal of Veterinary Science
基金
湖北省重点科技攻关项目 (2 0 0 1AA2 0 1B0 2 )
国家自然科学基金资助项目(3 0 2 0 0 11)
