摘要
目的 探讨醛固酮 (ALD)的致肝纤维化作用。方法 SD大鼠 90只 ,随机分成 3组。正常对照组 (8只 ) :正常饮食饮水 ,皮下注射花生油 ;模型组 (42只 ) :复合因素制成肝硬化模型 ;螺内酯干预组 (40只 ) :造模方法同模型组 ,螺内酯每天 10 0mg·kg-1灌胃。分别于第 2、4、6、8周末随机处死 8只大鼠 ,眼球采血并取肝右叶组织测定血清和肝组织ALD水平及胶原面密度。结果 大鼠血浆及肝组织ALD水平在模型组随肝纤维化加重同步升高 ,在螺内酯干预组较同期模型组明显升高 (P <0 .0 5 ) ,但肝内胶原面密度明显减少 (P <0 .0 5 )。结论 ALD对大鼠肝纤维化形成起促进作用 。
Objective To investigate the effect of aldosterone on the formation of hepatic fibrosis. Methods Ninety rats were randomly divided into three groups: normal control group consisting of 8 rats who were fed with normal food and water, model group consisting of 42 rats who were made into liver cirrhosis model by compound factors, and spironolactone prevention group consisting of 40 rats who were given spironolactone 100 mg·kg -1 into stomach per day when made into liver cirrhosis. At the end of week 2, week 4, week 6, week 8, 8 rats were randomly sacrificed. Blood was taken from the eyes and the liver tissues were taken from the right liver lobe. The level of ALD in the blood and liver tissues were measured. The contents of collagen in the liver tissues were also examined. Results In model group, the level of ALD in the blood and liver tissues rose as the hepatic fibrosis developed. ALD level in the spironolactone group was much higher than that in the model group (P< 0.05), but collagen percentage in the spironolactone group was less than that in the latter group (P< 0.05). Conclusion ALD can promote the formation of liver fibrosis in rats. Spironolactone may prevent hepatic fibrosis by acting against ALD receptor.
出处
《西安交通大学学报（医学版）》
CAS
CSCD
北大核心
2003年第5期458-460,共3页
Journal of Xi’an Jiaotong University（Medical Sciences）
关键词
醛固酮
肝纤维化
动物实验
发病机制
aldosterone
spironolactone
hepatic fibrosis
hepatic stellate cell
extracellular matrix
