摘要
目的 探讨白细胞介素 (Interleukin ,IL) 1基因家族中IL 1β 31/ 5 11位点、IL 1RN可变数目重复序列 (VNTR)的多态性及其与胃癌发病风险的关系。方法 使用变性高效液相色谱(DHPLC)对来自山东省临朐县胃癌高发现场人群与北京肿瘤医院标本库部分临床标本进行病例对照研究。PCR反应扩增目的片断 ,DHPLC分析中 ,采用非变性按片段长度分离双链DNA法分析IL 1RNVNTR基因型。采用部分变性法分析IL Iβ 31/ 5 11位点基因型 ,结果测序证实。 结果 IL 1β 31基因型多态和IL 1RN基因VNTR ,在弥漫型和肠型胃癌中 ,均不增加胃癌发病风险。IL 1β 5 11位点的T携带子在高发现场组胃癌中 ,增高胃癌发病风险 (OR =5 4 14 ,95 %CI :3 0 38～ 9 6 4 7)。T携带子在肠型胃癌中增高胃癌发病风险 ,OR =3 833( 95 %CI:2 2 82～ 6 4 38)。男性的T携带子在弥漫型胃癌中增高胃癌发病风险OR =3 4 6 4 ( 95 %CI :1 394～ 8 6 0 8)。对照组分为慢性萎缩性胃炎(CAG)、肠上皮化生 (IM )、异型增生 (DYS)三组 ,IL 1β 31基因多态性、IL 1RNVNTR在不同的病变阶段中的分布差异无显著意义 ,IL 1β 5 11位点在各病变阶段的T携带子基因型分布差异也无显著意义 ,均可明显增加现场组胃癌的发病风险。结论 IL 1β 31基因型和IL
Objective To investigate the correlation between polymorphisms of interleukin 1β and RN genes and risk of gastric carcinoma. Methods PCR and denaturing high performance liquid chromatography (DHPLC) were used to analyze the IL 1β 31 and 511 C/T polymorphisms and to genotype the IL 1RN penta allelic variable number of tandem repeats (VNTR):of the DNA from the peripheral blood of 143 patients of gastric carcinoma, 97 from Linqu County, Shandong Province, and 46 cases from the specimen bank of Beijing Cancer Hospital, and 337 controls without gastric carcinoma from Linqu County, most of which suffered from other gastric diseases. Results IL 1 511 polymorphisms (carriers of IL 1β 511T) were correlated with the increased risk of interstitial gastric carcinoma in both sexes ( OR = 3.833, 95% CI : 2.282~6.439) and increased risk of diffuse gastric carcinoma in males ( OR =3.464, 95% CI : 1.394 ~ 8.608). The frequencies of IL 1β 31 polymorphisms and IL 1IRN VNTR were not different between the gastric carcinoma group and the control group, IL 1β 511 T carrier could be seen in the controls at different precancerous stages, for chronic atrophic gastritis ( OR = 5.164, 95% CI : 2.661~10022), for interstitial metaplasia ( OR = 5.093, 95% CI : 2.708~9.463), and for dysphasia ( OR = 8.438, 95% CI : 3.939~18.073). Conclusion IL 1β 31 genotypes and. IL 2RN VNTR do not increase the risk of gastric carcinoma. The IL 1β 511 T genotype increases the risk of gastric carcinoma in every precancerous stage.
出处
《中华医学杂志》
CAS
CSCD
北大核心
2003年第17期1479-1483,共5页
National Medical Journal of China
基金
国家"8 63"高技术研究发展计划基金资助项目(2 0 0 1AA2 2 710 1)
北京市科委科技计划重大项目(H0 2 0 92 0 0 3 0 2 90 )
北京市科委"重大疾病遗传资源库的建立"项目基金 (95 5 0 2 13 2 0 0 )
