摘要
以光适应和暗适应条件下的罗氏沼虾复眼视网膜为材料 ,分别用高钙溶液、生理溶液、低钙溶液孵育后 ,用蛋白质提取液提取可溶性Gq蛋白α亚基并SDS PAGE电泳 ,利用TanonGIS凝胶图像处理系统分析其含量。从光暗条件下 3种溶液孵育后的感光细胞中都提取到了 4 2kDa的可溶性Gq蛋白α亚基。高钙溶液、生理溶液、低钙溶液孵育后的感光细胞中可溶性Gq蛋白α亚基的含量 ,光适应组分别是 3 13%、 3 0 9%、3 0 3% ;暗适应组分别是 3 17%、 3 0 6 %、 3 0 1%。在生理溶液和低钙溶液中 ,该蛋白含量光适应组大于暗适应组 ,但低钙溶液的光适应组与暗适应组无显著差异 ;在高钙溶液中 ,暗适应组显著大于光适应组 ,可能与钙离子反馈调节作用有关。
After light and dark adaptation,the retina of the Macrobrachium rosenber was respective incubated in high calcium solution,physiological solution and low calcium solution.Soluble Gq α was extracted from the retina of the Macrobrachium rosenber with protein extract and was electophoresised by SDS-PAGE.The percent of the soluble Gq α was analyzed by Tanon GIS gel image disposal system.After the incubation in three solutions,the soluble Gq α in 42?kDa was all extracted from photoreceptor cell.The percent of the soluble Gq α in photoreceptor cell in high calcium solution,physiological solution and low calcium solution was 3.13%,3.03% respectively on light adaptation and was 3.17%,3.06% and 3.01% respectively on dark adaptation.In physiological solution and low calcium solution,the percent of the protein on light adaptation was higher than that on dark adaptation,but in low calcium solution,the percent of the protein on light and dark adaptation was no significantly different;in high calcium solution,the percent of the protein on dark adaptation was higher than that on light adaptation.This was probably relevant to feedback regulation.
基金
上海市高等学校科学技术发展基金资助项目 ( 0 3DZ0 5 )
关键词
罗氏沼虾
感光细胞
钙离子
光适应
暗适应
可溶性Gq蛋白α亚基
Macrobrachium rosenberg
Photoreceptor cell
Soluble Gq protein α subunit
Calcium
Light adaptation
Dark adaptation
