摘要
采用随机扩增多态性DNA (RAPD)技术 ,对泥蚶在福建以南 (广东汕头和湛江 )和福建以北 (浙江温岭和韩国 )种群 (分别合称南方类群和北方类群 )做了遗传分化研究。由筛选出的 2 0个随机引物共获得10 3个清晰可辨的RAPD标记 ,扩增片段长度在 2 5 0～ 2 5 0 0bp。汕头种群与湛江种群 ,韩国种群与温岭种群之间的最小遗传距离分别为 0 0 6 12和 0 0 6 92 ,而南、北类群间的遗传距离却在 0 32 6 1～ 0 4 5 11。类群间近交系数也大于类群内。NJ和UPGMA法构建的系统树均显示汕头种群、湛江种群首先聚在一起 ,再与温岭种群和韩国种群聚合 ,说明两个类群发生了较明显的遗传分化 ,估计与地理隔离有关。
The random amplified polymorphic DNA (RAPD) technique was applied to analyze intra-species differentiation among Tegillarca granosa populations distributed to the south and north of Fujian province.Samples were collected from the south (Shantou and Zhanjiang in Guangdong province) and the north (Wenling in Zhejiang province,and Korea),and defined as the representatives of the southern populations (SP),and northern populations (NP),respectively.Amplified with 20 random primers, we acquired 103 distinguished RAPD loci,ranging from 250 bp to 2 500 bp in size.The least genetic distance between SP and NP was 0.3261-0.4511,while that within the populations of NP or SP was only 0.0612-0.0692.The inbreeding coefficient (Fst) between SP and NP was also larger than that within SP and NP.Using NJ and UPGMA methods,we also found the nearest phylogenetic relationship consistently occurred within the populations of the SP or NP.A significant intra-species genetic differentiation has occurred between SP and NP,probably due to the geographical isolation between the populations.
基金
浙江省科技厅重点项目 ( 0 0 110 3 2 0 7)
关键词
泥蚶
RAPD
温岭种群
韩国种群
汕头种群
湛江种群
亲缘关系
Tegillarca granosa
RAPD
Wenling population
Korea population
Shantou population
Zhanjiang population
Phylogenetic relationship
