摘要
水稻 91FS是一个稳定遗传的籼粳型不育突变体 ,大约 2 5 %的受精卵能正常发育成胚 ,约 75 %的受精卵核仁不融合 ,受精卵不再分裂 ,导致不育和不结实 ,育性六代稳定遗传 ,受精卵致死时空位点发生在精细胞进入卵细胞完成受精作用后。开花前后 ,91FS、籼粳杂种可育材料 91FM和以 91FS为父本的杂种F1代的花药、颖壳、叶片和子房的可溶性蛋白质SDS PAGE和IEF/SDS PAGE分析表明 ,91FS、91FM和F1代的蛋白质谱带多代保持稳定 ,3个材料间蛋白质谱带的异同 ,既表明了它们在遗传和发育上的某些共同功能和相互关系 ,又表明不同品种、器官和组织在遗传和发育上的某些特异性 ;91FS的子房在开花 1～ 2天 ,具有一条Mr=15 8.5× 10 3 的特有蛋白带 ,结合 91FS受精卵发育的细胞学和胚胎学时空定位 ,表明它们极有可能与 91FS稳定遗传的受精卵发育的时间和空间表达有关 .图 4表 1参
FS is a novel Indica-Japonica rice hybrid and heritable female-sterile mutant. Our previous embryo analysis showed that inside 91FS ovaries during anthesis days, only about 25% of fertilized eggs developed into normal mature embryos, which led to 91FS sterility and low setting rate. This unique fertility of 91FS has been heritable within six generations so far, and the lethal spatial-temporal site was found in the fertilized eggs. In this experiment, the soluble proteins from the ovaries, anthers, glumes, and leaves of 91FS, 91FM (a fertile Indica-Japonica rice hybrid) and F1 generation (91FS as male parent) before and during the anthesis days were extracted for SDS-PAGE and IEF/SDS-PAGE analysis. The protein electrophoretograms of the three materials were found stable within six generations. The identity and difference of the electrophoretograms indicated that these materials owned their common developmental characteristics as well as specific fertility. Interestingly, there was a unique protein 91FSa (M r = 158.5×103) found from 91FS ovaries that existed only on the first and second day of anthesis. 91FSa is probably related to the spatial-temporal expression of 91FS's stable fertility development. Fig 4, Tab 1, Ref 16
出处
《应用与环境生物学报》
CAS
CSCD
2003年第5期471-473,共3页
Chinese Journal of Applied and Environmental Biology
基金
中国科学院"九五"基础性研究重点项目 (KJ982 JI 62 4 )
国家自然科学基金资助项目 (No .39570 4 68)
国家计委科技攻关课题 (No .85 72 2 2 5 0 1 )
四川省重点科学技术课题资助项目~~
关键词
籼粳稻杂种
不育突变体
育性发育
空间表达
时间表达
Indica-Japonica rice hybrid
sterile mutant
fertility development
spatial-temporal expression
