摘要
目的 观察异丙酚和地西泮对氯胺酮诱导的大鼠大脑皮质Glu(谷氨酸)释放和c-fos基因表达的影响,探讨异丙酚抑制氯胺酮中枢神经系统副作用的基因机制。方法 将28只Wistar大鼠随机分为生理盐水3 ml-生理盐水1 ml组,生理盐水3 ml-氯胺酮 100 mg/kg组,异丙酚100 mg/kg-氯胺酮100 mg/kg组,地西泮10 mg/kg-氯胺酮:100 mg/kg组。于用药后30 min,检测c-fos mRNA水平。另取大鼠,用药注射后2 h取大脑皮质检测Glu含量、组织含水量和Fos蛋白阳性细胞率。结果 氯胺酮注射后30 min引起大脑皮质c-fos mRNA表达增高,2 h时大脑皮质Glu、水含量和Fos阳性细胞率与生理盐水组相比增加(P<0.01)。异丙酚和地西泮预处理抑制了c-fos基因表达以及组织谷氨酸、水含量的增加,且抑制程度不同。结论 c-fos基因参与了异丙酚和地西泮抑制氯胺酮引起的精神症状和神经元损害的基因机制;异丙酚抑制作用强于地西泮。
Objective To investigate the effect of propofol on C-fos expression and glutamate concentration in rat cerebral cortex induced by ketamine. Methods Twenty-eight male Wistar rats weighing 260-280 g were randomly divided into four groups of seven animals: group 1 received normal saline intraperitoneally (ip) (group NS); group 2 received NS + ketamine 100mg·kg-1 ip (group K); group 3 received propofol 100 mg·kg-1 + ketamine 100mg·kg-1 ip (group PK); group 4 received diazepam 10mg·kg-1 + ketamine 100 mg·kg-1 ip (group DK). The interval between the two intraperitoneal injections was 5 min in each group. The animals were decapitated 30 min after ip injection. C-fos mRNA expression was detected by RT-PCR method and fos protein expression by immuno-histochemical technique. Another forty Wistar rats were randomly divided into 4 groups of 10 animals as was described above. Two hours after ip injection, five animals in each group were decapitated for microscopic examination and the other five animals for determination of water and glutamate content of cerebral cortex.Results C-fos mRNA expression increased at 30 min after intraperitoneal ketamine. Ketamine induced significant increase in Fos protein expression, and glutamate and water content in cerebral cortex 2 h after ip injection. Propofol and diazepam inhibited the increases induced by ketamine ( P < 0.01 or 0.05) .Conclusion C-fos gene is involved in the mechanism by which propofol and diazepam inhibit the psychotomimetic symptoms and neuronal damage induced by ketamine.
出处
《中华麻醉学杂志》
CAS
CSCD
北大核心
2003年第10期764-766,共3页
Chinese Journal of Anesthesiology
