摘要
目的 构建伯氏疏螺旋体PD91 菌株外膜蛋白C(OspC)的表达载体 ,克隆表达OspC ,用于莱姆病的预防、诊断和致病机理上的研究。方法 用PCR扩增PD91 ospC基因 ,定向克隆到表达载体PET -11D ,构建重组质粒。采用酶切分析及序列测定等方法鉴定重组质粒的正确性。结果 ospC基因被正确克隆到表达载体PET -11D中。序列测定结果证实与已报道的ospC基因序列同源性介于 62 %～ 86%之间。结论 我国PD91 菌株的ospC编码基因与已报道菌株的ospC菌株在同源性上存在较大的差异。PET -11D -ospC重组质粒的成功构建为我国莱姆病的进一步研究奠定了基础。
Objective To construct an expression vector carrying the outer surface protein C gene of Borrelia burgdorferi which was cloned and expressed for studies in prevention,diagnosis and pathogenic mechanism of Lyme disease. Methods The ospC gene was amplified from the genome of Borrelia burgdorferi PD 91 strain by PCR and recombined with plasmid PET-11D. The recombinant plasmid PET- 11D-ospC was identified with restriction endonuclease analysis and sequencing. Results The ospC gene was cloned correctly into vector PET-11D. The homology of nucleotide sequence of the inserted fragment in the plasmid was between 62% to 86% compared with the published sequence of ospC gene. Conclusion The homology of PD 91 strain presented highly difference with foreign isolates, and the PET- 11D-ospC constructed successfully pave the way for the research of Lyme disease.
出处
《中国媒介生物学及控制杂志》
CAS
CSCD
2003年第5期329-331,共3页
Chinese Journal of Vector Biology and Control
基金
国家自然科学基金资助项目 (31 0 70 82 0 )
关键词
伯氏疏螺旋体
外膜蛋白C
克隆
同源性
Borrelia burgdorferi
Outer surface protein C
Cloning and expression
Homology
