摘要
目的 采用HPLC法测定绿药膏中盐酸林可霉素的含量。方法 用YWG -C18柱 (2 0 0mm× 4 .6mm ,10 μm) 0 .0 5mol/L硼砂溶液 (用 85 %H3 PO4调 pH值至 6 .0 )∶甲醇 (4∶6 )为流动相 ,检测波长为 2 14nm。结果 林可霉素线性范围为 0 .2 5 2 6～ 2 .0 2 0 8mg/ml,r =0 .9999,回收率为 99.5 % ,RSD =0 .8% (n =5 )。结论 本法简便、灵敏、准确 。
Objective To establish an HPLC mathod for the Lincomycin hydrochloride in Lu Yao Gao.Methods The HPLC system consisted of a YWG-C 18 colum (200 mm×4.6 mm,10 μm) and 0.05 mol/L borax(containing 85% H 3PO 4,pH 6.0) methanol (4∶6) as the mobile phase.The detection wavetength was 214 nm.Results The linear range was 0.2526～2.0208 mg/ml,r=0.9999;the average recoveries was 99.5%,RSD=0.8% (n=5).Conclusion The method was simple,sensitive and reproducible,and can be used for the quality control this preparation.
出处
《广东药学》
2003年第5期19-21,共3页
Guangdong Pharmaceutical Journal
