摘要
目的:研究肌损伤浸液作用于骨髓间充质干细胞(mesenchymalstemcells,MSCs)对其增殖的影响以及成肌诱导作用。方法:分离、扩增小鼠骨髓MSCs,以切割伤及液氮冻伤法建立肌损伤并提取伤后8～72h的伤浸液,以Bradford比色法检测其蛋白含量并选取含量最高的时相组,以四唑盐(MTT)法检测该组伤浸液对MSCs增殖的影响,以反转录聚合酶链反应(reversetranscription-polymerasechainreaction,RT-PCR)法检测其作用后生肌调控因子中生肌素(myogenin)的表达。结果:肌损伤后12h时的伤浸液蛋白含量最高;作用于MSCs后可明显增强MSCs的增殖能力,但并不能诱导MSCs表达myogenin。结论:肌损伤浸液可促进MSCs的增殖但对MSCs无成肌诱导分化作用。
AIM:To study the influence of muscle-injury wound fluid on the proliferation and myogenic differentiation ability of bone marrow-derived mesenchymal stem ce lls (MSCs). METHODS: Bone marrow-derived MSCs of mouse were seperated and purified; muscl e injury were created by using incising injury and freezing injury with liquid n itrogen and wound fluid was collected in the 8-72 hours after damaged. Their pr oteins concentration were measured with Braford colorimetry method and choosed t he higest concentration group; the influence of this group wound fluid on the pr oliferation of MSCs was detected with microculture tetrozolium(MTT) method; the expression of myogenin on MSCs was detected with reverse transcription-polymera se chain reaction(RT-PCR) method. RESULTS:The higest concentration of wound fluid was at the 12 hours, after eff ected on MSCs it could enhance the proliferation ability of MSCs but could not i nduce MSCs to express myogenin.
出处
《中国临床康复》
CSCD
2003年第26期3552-3553,共2页
Chinese Journal of Clinical Rehabilitation
基金
"973"国家重点基础研究发展规划资助项目(G1999054205)~~
关键词
骨髓间充质干细胞
肌损伤浸液
细胞增殖
成肌诱导作用
创伤修复
肌损伤
Muscle-injury wound fluid can promote MSCs proliferation but have no effect on inducing MSCs to differentiate into myoblasts.
