摘要
目的:评价胶原蛋白(Collagen)的细胞相容性;研究胶原蛋白(C)与大鼠胎脑神经细胞(FBN)复合体(C-FBN)脑内移植对脑损伤的修复作用。方法:将大鼠FBN与胶原蛋白联合体外培养,进行光镜、扫描电镜观察。并将体外联合培养3～5d的C-FBN(移植前48h培养液中加入BrdU)移植到大脑皮质损伤模型的大鼠脑损伤部位,术后3,6,10,15及30d光镜、BrdU免疫细胞化学染色(ICC)、透射电镜观察脑组织对移植物的反应、移植细胞生长状态及载体在体内的降解情况等。结果:胶原蛋白对FBN生长无不良影响;脑组织对移植物无明显的免疫排斥反应;胶原蛋白与周围脑组织整合好,有血管长入移植物中;载体内有大量存活神经细胞,而且术后各时段都检出BrdU阳性细胞;移植区有突触形成;胶原蛋白海绵吸收、降解快,可诱导组织再生。结论:胶原具有良好的细胞相容性和组织相容性,是神经组织工程的一种较为理想的生物载体材料;生物材料与胎脑神经细胞联合培养移植是治疗脑损伤较有前途的新方法。
AIM:To evaluate the biocompatibility of collagen sponges and study the repair effect that transplanting the collagen sponges combined with cultured fetal brai n neurocytes (C-FBN) to injured brains of rats. METHODS:FBNs were seeded on collagen sponges and cultured in vitro, cell compa tibility was detected by light microscope and scanning electronic microscope (SE M). the collagen sponges combined with FBNs cultured for 3-5 d (marked with Brd U at 48 h before transplatation) were transplanted into the injured rat brains a nd the morphological characters were detected by light microscope,electronic mi croscope, immunocytochemical stain (ICC) and transmission electron microscopy (T EM) at 3,6,10,15 and 30 d after operation. RESULTS:FBN could attach to and extend on collagen sponges. No immunological r ejection was obviously found after operation. Collagens were perfectly integrate d with peripheral brain tissue and blood vessels were seen in the graft. The neu rocytes were of massive survival within the carrier and Brdu positive cells were seen at different stages after operation.Synaptics were found in the transplant ed area. The collagen sponges with the fast absorption and degradation could ind uce tissue regeneration.
出处
《中国临床康复》
CAS
CSCD
2003年第25期3430-3431,T001,共3页
Chinese Journal of Clinical Rehabilitation
关键词
脑损伤
胶原蛋白
胎脑神经细胞
移植
动物实验
Collagen sponge with the good cell compatibility and histocompatibi lity is an ideal carrier material for tissue engineering. The Transplantation of collagen sponges combined with cultured FBN into damaged brain is a good method for surgical tr
