摘要
目的探讨乙型肝炎病人血清HBVDNA含量与临床特征的关系。方法对临床确诊的 35 6例乙型肝炎病人检测血清HBVDNA、HBVM、肝功能 ,其中 5 8例病人作了肝活检 ,对其肝脏组织进行HE及Masson染色 ,观察肝组织病理特征 ,并对不同特征的HBVDNA水平进行比较。结果血清HBVDNA含量HBeAg阳性组与阴性组比较差异具有极显著性 ;ALT正常组与不正常组之间差异具有显著性 ,而ALT不同水平组之间无明显差异 ;肝脏病理炎症分级G0 ～G1组与G2 、G3 及G4之间均有显著性差异 ,而G2 、G3 及G4三组之间比较无明显差异 ;纤维化分期S0 ～S1组与其他三组均有显著性差异 ,S2 与S3 组间无明显差异 ,S2 、S3 组与S4组比较差异有显著性。结论HBVDNA仅代表血清HBV复制的水平 ,不能反映肝脏炎症的严重程度 。
ObjectivesTo explore the correlation between the serum levels of HBV DNA and clinical features in patients with viral hepatitis B.MethodsLiver functions, Hepatitis B markers (HBVM) and serum levels of HBV DNA were detected in three hundred and fifty six patients with chronic hepatitis B whose clinical diagnosis were confirmed. Liver biopsy was performed in 58 patients, their liver tissues were stained by HE and Masson stains to observe the pathological characteristics. HBVM and HBV DNA were assayed by ELISA and fluorescence ration PCR separately. HBV DNA levels in patients showing different clinical features were compared.ResultsThe serum levels of HBV DNA in HBeAg positive group revealed very significant difference as compared with those in HbeAg negative group. Serum HBV DNA levels were correlated to HbeAg and not markedly to the liver pathology and liver function. Obvious difference existed between the group with normal activity of alanine aminotransferase(ALT) and group with abnormal ALT activity. As for the pathological grades of hepatitis, significant differences were also found among G 0～G 1 groups and G 2 , G 3 and G 4 groups. S 0～S 1 groups of cirrhotic stage differed distinctly from S 2, S 3 and S 4 groups, whereas there were significant discrepancies between S 2/S 3 and S 4 groups.ConclusionHBV DNA only represents the replication level of serum HBV and can not reflect the severity of hepatitis. The replication levels of HBV decrease during early liver cirrhosis.
出处
《医学临床研究》
CAS
2003年第9期676-678,共3页
Journal of Clinical Research
