摘要
目的 探讨先天性心脏病房间隔缺损 (房缺 )患者心肌组织与正常人心肌组织基因表达谱系的差异 ,初步分析房缺患者心肌组织基因表达谱系的特征。方法 提取房缺患者及正常人心房肌组织mRNA ,标记cDNA探针 ,分别与含有 180 0 0个人类基因片段的cDNA微矩阵 (cDNAmicroarray)芯片杂交 ,对二者差异表达基因进行生物信息学分析。结果 ( 1)获得表达差异 2倍以上基因数 10 5 6个 ,其中 3倍以上 2 6 4个 ,5倍以上 46个 ;( 2 )差异表达基因涉及发育、分化、代谢、转录调节、信号转导、细胞周期等不同功能的基因群。结论 房缺患者心肌组织的基因表达谱存在Wnt信号途径发育不良、细胞外基质信号增强和细胞内信号传导减弱、细胞周期调控异常、胎儿转录程序 (fetaltranscriptionprogram)启动、高能量需求、细胞骨架紊乱和心肌收缩力降低等特征。
Objective To investigate the difference in gene expression profiles between myocardium of atrial septal defect (ASD) and normal myocardium using cDNA microarray, and to analyze the feature of gene expression profiles in human ASD myocardium. Methods The cDNA probes prepared from mRNA of ASD myocardium and normal myocardium were hybridized with two identical cDNA microarray membranes containing 18 000 human genes. The functions of the differentially expressed genes were analyzed by bioinformatics. Results 1 056, 264 and 46 genes were found significantly differentially expressed over 2,3 and 5 folds respectively between ASD myocardium and normal myocardium. Different clusters of genes associated with embryo development, cell differentiation, metabolism, DNA transcription, signal transduction, cell cycle, etc were concerned. Conclusion Impaired signal transduction including Wnt signal pathway, dysregulation of cell cycle, initiation of fetal transcription program, higher requirement of energy, derangement of cell skeleton, and reduced contraction capacity of myocardium, etc are the features of gene expression profiles of human ASD myocardium.
出处
《中华心血管病杂志》
CAS
CSCD
北大核心
2003年第3期180-183,共4页
Chinese Journal of Cardiology
基金
江苏省自然科学基金资助项目 (BK2 0 0 10 43)
