摘要
以东海宁波海区收集的甲壳内侧出现白斑为特征的发病中国对虾为材料,从中分离出一株病毒(ZJ2001),经电镜和动物试验鉴定为对虾白斑综合征病毒;用煮沸法一步获得病毒基因组DNA,以此基因组为模板,利用PCR技术,扩增病毒囊膜蛋白VP19的基因,将扩增得到的基因克隆并测序.用DNATools软件对测得的序列分析表明:VP19基因序列与GeneBank中的比较(AF309029),其核苷酸同源性为99%,氨基酸同源性为98%.
White spot syndrome virus (WSSV) is a virus infecting shrimp and other crustaceans, which is unclassified taxonomically. WSSVinfected penaeid shrimp, P. chinensis, with typical white spots on the inside carapace of the head were collected from Ningbo (Zhejiang, East China Sea) of China. The WSSV was identified and named ZJ2001. In addition, the major envelope protein VP28 of the virus genome DNA, which was gained by ferv, was amplified by PCR. The production of PCR then was cloned and sequenced. The generated sequences were analyzed with DNA Tools software and the result revealed that the sequence of VP19 gene were compared with GeneBank (AF309029) and it shows a high degree of homology of amino acids (99%)
出处
《科技通报》
北大核心
2003年第6期473-476,共4页
Bulletin of Science and Technology
基金
江苏省高校自然科学研究计划项目(03KJB240125)
