摘要
目的探讨RhoC基因与肝细胞癌 (HCC)的发生发展和侵袭转移的关系。方法采用逆转录聚合酶链反应 (RT PCR)在半定量水平检测 2 5例HCC及其相对应的癌旁肝组织和 4例门静脉主干癌栓或肝外转移灶中RhoCmRNA的表达 ;同时采用PCR产物单链构象多态性 (PCR SS CP)银染法检测RhoC基因的突变情况。结果本组 2 5例HCC中RhoCmRNA的表达较癌旁肝组织高 ,其光密度比值分别为 1 8± 1 1和 1 0± 0 7,P <0 0 1,差异有显著意义 ;4例门静脉主干癌栓或肝外转移灶中的RhoCmRNA的表达较相对应的肝内肝癌病灶高 ,其光密度比值分别为 3 3± 0 5和 2 0± 0 7,P <0 0 1,差异有显著意义 ;将 2 5例HCC分为RhoC基因过表达组和高表达组后发现 ,过表达组中肝癌细胞分化较差 ,静脉受侵严重 ,且以结节性肝癌居多 (P <0 0 5 ) ;而 2 5例HCC经PCR SSCP银染法分析未发现异常泳动条带。结论RhoC基因与HCC的发生发展和侵袭转移密切相关 ,且可能是通过表达升高而非突变发挥作用。
ObjectiveTo investigate the expression of RhoC gene in primary hepatocellular carcinoma and to evaluate the relationship between RhoC gene expression and invasion and metastasis of primary hepatocellular carcinoma.MethodsThe mRNA expression of RhoC gene was examined by polymerase chain reation after reverse transcription (RT-PCR) in 25 cases of primary hepatocellular carcinoma (HCC) and adjacent non-cancerous tissuse. In addition, the mutation of RhoC gene was examined by polymerase chain reaction-single strand conformational polymorphism(PCR-SSCP)ResultsThe mRNA expression of RhoC in tumor tissue were higher than that in adjacent liver tissue,1.8±1.1 vs. 1.0±0.7( P <0.01). The metastatic lesions showed significantly higher RhoC mRNA levels than corresponding tumor tissue in the liver ,3.3±0.5 vs. 2.0±0.7( P <0.01).There was close relationship between RhoC gene expression and clinicopathological findings, including cell differentiation, vein invasion, numbers of tumor node and metastatic lesion. Mutation was not found in RhoC gene by PCR-SSCP examination.ConclusionsThe RhoC gene may be related to malignant transformation and development of HCC probably by overexpression.
出处
《中华普通外科杂志》
CSCD
北大核心
2003年第9期558-560,共3页
Chinese Journal of General Surgery
基金
国家"十五"科技攻关基金资助项目 (2 0 0 1BA70 3B)
湖南省计委资助项目 (2 0 0 1 90 7)
