摘要
背景:葡聚糖硫酸钠(DSS)可诱导大鼠结肠炎,但其发病机制尚不完全清楚。目的:探讨DSS诱导大鼠结肠炎的发病机制。方法:16只健康雄性Sprague-Dawley大鼠随机分成DSS模型组(10只)和正常对照组(6只),DSS模型组大鼠自由饮用2％DSS溶液8天,正常对照组大鼠正常饮水。于第9天处死大鼠,采用酶联免疫吸附测定(ELISA)方法测定血清肿瘤坏死因子(TNF)-α和白细胞介素(IL)-6水平,采用免疫组化方法测定结肠黏膜细胞间黏附分子(ICAM)-1的表达和核因子(NF)-κB活性。结果:DSS模型组大鼠出现腹泻、便血以及结肠黏膜糜烂或溃疡,血清TNF-α和IL-6水平以及结肠黏膜ICAM-1表达和NF-κB活性均较正常对照组显著增高(P<0.01)。结论:在DSS诱导的大鼠结肠炎中,DSS可能通过活化NF-κB使TNF-IL-6和ICAM-1生成增加,导致结肠黏膜炎性损害和腹泻、便血。
Background: Dextran sulfate sodium (DSS) can induce colitis in rats, but its mechanism is not fully understood. Aims: To investigate the pathogenesis of DSS-induced colitis in rats. Methods: Sixteen healthy male Sprague-Dawley rats were randomized into DSS model group (10 rats, drinking a solution of 2% DSS for 8 days) and control group (6 rats, drinking only water). All rats were killed on day 9. The serum levels of tumor necrosis factor (TNF)-α and interleukin (IL)-6 were determined by enzyme-linked immunosorbent assay (BLISA), and the expression of intercellular adhesion molecule (ICAM)-l and the nuclear factor (NF)-κB activity in colonic mucosa were detected by immunohistochemistry. Results: Diarrhea, bloody stool and erosion or ulceration of colonic mucosa were found in DSS model rats. The serum levels of TNF-α and IL-6 as well as the expression of ICAM-1 and NF-κB activity in colonic mucosa of DSS model rats increased significantly as compared with those in the controls (P< 0.01). Conclusions: The production of TNF-α, IL-6 and ICAM-1 increase through activation of NF-κB in DSS-induced colitis in rats, and as a result, leading to the colonic mucosal inflammatory lesions, diarrhea and bloody stool.
出处
《胃肠病学》
2003年第5期283-286,共4页
Chinese Journal of Gastroenterology
基金
本课题由上海市教委项目(No.00B07)
上海市重点学科建设项目资助
