摘要
目的 :研究中药软肝冲剂对肝纤维化大鼠 p2 1m RNA表达及细胞周期各时相 DNA含量的影响 ,探讨软肝冲剂促进肝细胞再生、阻止肝纤维化、肝硬化形成的作用机制。方法 :采用 RT- PCR及流式细胞计数仪分别检测 CCl4 肝纤维化大鼠 p2 1m RNA表达、细胞周期各时相 DNA含量。结果 :软肝冲剂有降低肝纤维化大鼠 p2 1m RNA含量的作用 ,与模型组比较差异有显著性意义 (P <0 .0 1) ,在实验的第 12天及 4 5天 ,大剂量组疗效明显优于小剂量组 (P <0 .0 1) ,且呈一定的量效关系 ;正常肝组织 p2 1m RNA呈相当低水平表达 ,其表达相对值为 (0 .17± 0 .0 6 ) U。定量 DNA分析发现模型组中处于 G1 期的细胞数要明显多于其他各组 (P <0 .0 1) ,软肝冲剂大剂量组中处于 G1 期的细胞数明显少于小剂量组 (在实验的第 12、 4 5天 ,均 P <0 .0 1)。结论 :软肝冲剂能调节 CCl4 肝纤维化大鼠 p2 1基因表达 ,调节细胞周期 ,促进肝细胞再生 ,抑制肝纤维化、肝硬化的形成。
Objective:To study the effects of Ruangan granule(RG) on p21 mRNA expression in the experimental hepatic fibrosis induced by Carbon Tetrachloride(CCl 4) in rats and explore its mechanism. Methods: RT-PCR and flow cytometer were used to test the expression of p21mRNA and the content of DNA of the hepatic cells of different phases in the SD rats with experimental hepatic fibrosis induced by Carbon Tetrachloride (CCl 4).Results: RG decreased the level of p21mRNA, which showed significant difference by comparison with hepatic fibrosis model groups(P<0.01). The effect of large dose group was better than that of the small dose group (P<0.05~0.01). The effect reinforced along with the increase of the dose. The expression level of p21mRNA in normal liver tissues was strikingly lower (0.17±0.06U). And DNA content analysis showed that percentage of cells in G1-phase of model group was much higher than that of the other groups. Conclusion: RG had definite effect of anti-hepatic damage, which was better than that of colchicines treatment group. The possible mechanism was to decrease the level of p21mRNA, regulate the cell cycle and promote regenesis of the hepatocytes so as to inhibit the formation of hepatic fibrosis and hepatocirrhosis.
出处
《中国中西医结合消化杂志》
CAS
2003年第5期267-269,共3页
Chinese Journal of Integrated Traditional and Western Medicine on Digestion
