摘要
目的 在神经细胞群的水平上研究脑干蜗核 (cochlearnucleus ,CN)和前庭核 (vestibularnucleus ,VN)神经元电活动。方法 自新生小鼠 (1～ 5天 )制备离体脑干切片 ,用吸光性电压敏感染料RH155染色 2 0分钟。采用光学记录膜电位 (opticalrecordingmembranepotential)技术 ,观察电刺激位听神经 (第 8颅神经 ,nVIII)后脑干CN和VN的神经电活动。结果 ①电刺激nVIII断端后光学记录显示兴奋传导至CN和VN核团 (n =40 ) ;②CN和VN神经兴奋有激发延迟 (onsetlatency)和高峰延迟 (peaklatency) ;③所记录的光学信号具有光吸收波长特性 ,表明光学记录的可靠性 ;④光学信号包括峰样快反应信号 (spike -likefastsignal)和持续较长时间的慢反应信号 (slowsignal) ;⑤连续刺激nVIII后发现慢反应信号大小递减 ,为突触疲劳 (synapticfatigue)现象。结论 本研究表明光学记录膜电位方法可以在神经细胞群的水平上直观观察脑干蜗核和前庭核神经电活动的时空二维方式及其兴奋性突触传递过程 。
Objective To observe the spatial and te mporal patterns of neuronal excitation in the population of neurons of cochlear nucleus (CN) and vestibular nucleus (VN). Methods Brainstem slices were prepared from postnatal 1～ 5 days mice and stained by RH155, an absorption voltage-sensitive dye, for 20 mi n. Using a multiple-side optical recording system, the optical imaging of the ev oked responses was gained in brainstem slice after vestibulocochlear nerve elect rical stimulation. Results The spatiotemporal patterns of excitatory propaga tion in CN and VN were illustrated with optical imaging. There was different ons et latency and peak latency in CN and VN, respectively. These optical signals in CN and VN were wavelength dependent and consisted of two components: the spike- like fast signal and long-duration slow signal.Conclusion The present study indicated the feasibility of using optical recording for revealing visually the synaptic transmission of aff erent input in CN and VN in brainstem, and the optical recording technique shoul d be a new and practical method for the neuronal electro-physiology in central n ervous system.
出处
《听力学及言语疾病杂志》
CAS
CSCD
2003年第4期277-280,T001,共5页
Journal of Audiology and Speech Pathology
基金
国家自然科学基金 (编号 30 0 0 0 1 89)
军队十五科研基金 (编号0 1Q0 50 )
解放军总医院"中青年学科带头人"人才工程课题基金资助
关键词
光学记录
电压敏感染料
蜗核
前庭核
神经电生理
Optical recording Voltage-sensitive dye Ccochlear nucleus Vestibular nucleus Neuronal electro-physiology
