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铜绿假单胞菌外毒素对淋巴细胞毒性作用 被引量：1

Cytoxicity exhibited by Pseudomonas Aeruginosa Extoxin A on Human Lymphocyte
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摘要 目的　探讨铜绿假单胞菌外毒素A(PEA)对人外周血单核细胞 (PBMC)的毒性剂范围及抗PEA血清对PEA的中和作用。 方法 　采集健康成年人的静脉血 ,密度梯度离心分离PBMC。不同浓度的PEA与PBMC37℃、5 %CO2 共同孵育 4 8h ,用台盼蓝染色排除法和四甲基偶氨唑蓝 (MTT)法检测细胞存活率。 结果　台盼蓝染色排除法和MTT法结果一致 ,PEA在 10 0 0ng/ml时PBMC的存活率显著下降 (P <0 0 1) ,PEA剂量在 0～80ng/ml时 ,对细胞存活率无明显影响 (P >0 0 5 )。而抗PEA抗体完全中和PEA的细胞毒作用。 结论　PEA在 10 0 0ng/ml时 ,对PBMC表现出细胞毒作用 ,而抗PEA血清可以中和PEA的毒性作用。
作者 邓予晖 李素珍 马艳艳
机构地区 河南省人民医院检验科 河南省出入境检验检疫局门诊部
出处 《医药论坛杂志》 2003年第18期13-14,共2页 Journal of Medical Forum
关键词 淋巴细胞 毒性作用 人外周血 单核细胞 铜绿假单胞菌外毒素A
分类号 R996.1 [医药卫生—毒理学]
  • 相关文献

参考文献7

  • 1梁小兵,庄汉澜,周洁,詹轶群,郭庆福.铜绿假单胞菌外毒素A的抗原性及其与辅助细胞的关系[J].中华微生物学和免疫学杂志,2000,20(5):447-450. 被引量：1
  • 2梁小兵,庄汉澜,周洁,詹轶群,郭庆福.铜绿假单胞菌外毒素A的生产、分离纯化和鉴定[J].生物技术通讯,2000,11(2):107-110. 被引量：9
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二级参考文献13

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共引文献8

同被引文献5

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医药论坛杂志
2003年 第18期

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