摘要
目的 探讨贵州汉族人群β_2-肾上腺素能受体(β_2-AR)16、27位点基因多态性与支气管哮喘的相关性。方法 采用等位基因特异性聚合酶链反应(AS-PCR)方法,对74例支气管哮喘患者、39例健康者进行β_2-AR基因多态性分析。结果(1)贵州汉族人群β_2-AR基因16、27位点多态性分布频率与英美高加索人群不同,与国内有关报道结果一致;(2)支气管哮喘组人群β_2-AR基因16位点多态性分布频率:杂合子基因型占47.2％,甘氨酸纯合子基因型占35.2％,与健康组比较,差异有显著意义(P<0.05),而等位基因频率与健康组比较,差异无显著意义(P>0.05);27位点多态性分布频率:谷氨酰胺纯合子基因型占52.7％,杂合子基因型占33.7％,谷氨酸纯合子基因型占13.6％,以及等位基因频率与健康组比较,差异无显著意义(P>0.05)。结论 贵州汉族人群中β_2AR基因16位点多态性与支气管哮喘相关联;27位点多态性与支气管哮喘无关联。
Objective To analyze the association betweenβ2-AR genetic polymorphism and asthma in the people of the Han nationality of Guizhou. Methods Allele Specific -PCR techniques were used to determine 16、27 loci ofβ2-AR genetic polymorphisms in 74 unrelated patients with asthma、39 healthy persons of the Han nationality. Results (1) The distribution frequency of genotypeβ2-AR 16 locus in asthmatics: Arg/Arg genotype accounts for 17. 6%, Arg/Gly 47. 2% and Gly/Gly 35. 2%. There were significant difference in the frequencies of Arg/Gly and Gly/Gly compared with control group. The frequency of polymorphismsβ2-AR 27 locus in asthmatics; Gln/Gln genotype accounts for 33.7%, Gln/Gln 52. 77% and Glu/Glu 13. 6%. Conclusion These results suggest that β2-AR 16 locus genetic polymorphism is correlated with asthma, 27 locus genetic polymorphism is not correlated with asthma in the people of the Han nationality of Guizhou.
出处
《贵州医药》
CAS
2003年第10期878-880,共3页
Guizhou Medical Journal
