摘要
目的 探索胰腺移植缺血 再灌注损伤 (I RI)的发生机制及防治措施。方法 采用双套管 +移植物腹主动脉三通管外接法完成大鼠全胰十二指肠同种异体移植动物模型。实验分为A、B组 ,每组 15只 ,分别于术后 6、2 4h处死。处死时检测非空腹血糖 ,并行移植物组织学观察。结果 3 0次实验中 ,总手术时间仅为 2h左右 ,移植手术成功率 10 0 %。排除因套管内血栓形成引起移植物失功的 3例外 ,其余受试大鼠血糖水平均在术后 6或 2 4h降至正常 ,移植胰功能存活率为90 % (2 7/3 0 )。A、B两组组织学改变符合I RI病生特点。结论 本模型操作简便、稳定 ,不需阻断体循环 ,具有术后移植物功能恢复早的优点 。
Objective To explore the mechanism and prevention ischemia reperfusion injury (I RI) of pancreas transplantation.Methods The animal model of whole pancreaticoduodenal allotransplantation was developed by using the technique of dual cannula combined with abdominal aorta connected with te.All recipient rats divided into group A ( n =15) and B ( n =15) were respectively sacrificed at 6 h and 24 h after transplantation to examine glucose level of non fasting blood samples and histological changes of graft.Results 30 operations were performed.The total procedure of transplantation lasted about 2 h.The successful rate of operation was elevated to 100%.Except 3 cases of graft non function because of thrombosis in cannula,the glucose level of the remaining recipients was reduced to normal level at posttransplantaion 6 h or 24 h.The survival rate of graft function was 90% (27/30).The histological changes of two groups were coincident with those characteristic of I RI.Conclusion It is a simple and stable model without blocking systemic circulation.This model could recover the function of graft in advanced.
出处
《中华实验外科杂志》
CAS
CSCD
北大核心
2003年第10期944-945,共2页
Chinese Journal of Experimental Surgery
