摘要
目的 :探讨环磷酰胺(cyclophosphamide,CP)对染色体损伤与P53基因表达间的关系。 方法 :以正常人外周血淋巴细胞为材料 ,用流式细胞仪检测环磷酰胺对突变型P53基因表达的影响 ,同时以微核(MN)及姐妹染色单体互换(SCE)为指标 ,进行致突变研究。 结果 :环磷酰胺用药组突变型P53基因表达率为30.81 % ,微核率为8‰ ,姐妹染色单体互换率为9.21±1.08 ,与对照组比较均有显著差异。 结论
Purpose: To study the relation between chromosome damage induced by cyclophosphamide(CP) and the expression of mutated P53 gene. Methods: The expression of mutated P53 gene in human lymphocytes was investigated using flow cytometry.And the micronucleus(MN) and sister chromatid exchange (SCE) tests were studied in vitro. Results: The expression ofmutated P53 gene in human lymphocytes treated with CP was 30.81 %,(P < 0.01)compared with the control .The frequencies of SCE and MN in human lymphocytes exposed with CP were 9.21±1.08 and 8 ‰ respectively(P < 0.01),compared with the control. Conclusion: The mutagenic effect of CP may be related to the expression of mutated P53 gene .
出处
《癌变·畸变·突变》
CAS
CSCD
2003年第4期228-230,共3页
Carcinogenesis,Teratogenesis & Mutagenesis
基金
江苏省教育厅自然科学基金资助项目(KJB360006)
