摘要
目的研究不同时期糖尿病大鼠实验性牙周炎组织病理学改变及牙周组织中TNF-α的变化,从细胞学角度探讨糖尿病促进牙周炎发生发展的机制。方法用链脲佐菌素给大鼠行腹腔一次注射,建立糖尿病大鼠模型;上下颌牙用丝线结扎法形成局部刺激因素。观察6周、9周、12周时大鼠牙周组织变化,并用免疫组织化学结合MSP定量分析法,检测牙周组织的TNF-α水平。结果①糖尿病大鼠较非糖尿病大鼠的实验性牙周炎发生时间早、病损严重;②糖尿病大鼠较非糖尿病大鼠牙周炎时牙周组织中TNF-α含量增加。结论①糖尿病在牙周炎发生发展中起促进作用;②糖尿病时,TNF-α释放增加,使组织损伤-修复的动态平衡失调,致牙周组织炎症加重。
Objective The histologic and TNF-αchanges of the experimental periodontitis were studied in diabetic rat model in different periods.The mechanism of the occurring and developing of periodontitis was probed on the aspect of cytology.Methods The diabetic rat models were established by injecting streptozotocin into its abdomen.The local stimulation was caused by tiring silk thread to teeth.The histologic changes of periodontium were observed at6,9,and12weeks.The level of TNF-αwas testified using immunohistochemistry method at12weeks.Results Periodontitis occurred in diabetic rats earlier than in non-diabetic ones.The destructions were more severe and more widely in diabetic rats than in non-diabetic ones.The level of TNF-αwas higher in the experimental periodontium of diabetic rats than those non-diabetics.Conclusions Diabetes mellitus has an accelerating effect on occurring and developing of periodontitis.When with diabetes,more cytokines were released,and cause the unbalance of ruin and repair and result in the enhancing of periodontitis.
出处
《上海口腔医学》
CAS
CSCD
2003年第5期352-355,共4页
Shanghai Journal of Stomatology
关键词
糖尿病
实验性牙周炎
免疫组织化学
肿瘤坏死因子-Α
Diabetes Mellitus
Experimental Periodontitis
Histology Immunohistochemistry
Tumor Necrosis Factorα
