摘要
目的 :研究氟康唑和伏立康唑对 2 14株酵母菌的体外抗菌活性。方法 :酵母菌收集自 2 0 0 0年 1月至 2 0 0 1年 12月住院患者提供的标本。采用酵母菌纸片扩散法敏感试验 ,Mueller Hinton琼脂中补充 2 %葡萄糖和 0 .5mg/L美蓝 ,其他类似于美国国家临床实验室标准委员会 (NCCLS)M2 A6细菌药敏试验法。采用BIOMIC仪自动读取平皿抑菌环直径、解释和记录试验结果及核对质控数据。结果 :白念珠菌最常见 ,占 5 4 .7% ,其次为热带念珠菌占 18.7% ,光滑念珠菌占 12 .1% ,近平滑念珠菌占 4 .7% ,克柔念珠菌占 3.7%和新型隐球菌占 3.7%。白念珠菌、热带念珠菌和近平滑念珠菌对氟康唑的敏感率为 92 .5 %～10 0 .0 % ,MIC90 均在敏感范围内 (<3～ 8mg/L) ,伏立康唑对白念珠菌、热带念珠菌、光滑念珠菌和近平滑念珠菌的MIC90 为 0 .0 6 4～ 1.5mg/L。结论 :氟康唑对白念珠菌、热带念珠菌和近平滑念珠菌有较好的抗菌活性 ,伏立康唑比氟康唑对白念珠菌、热带念珠菌、光滑念珠菌和近平滑念珠菌有更高的抗菌活性 。
Objective: To investigate and compare the in vitro activities of fluconazole and voriconazole against 214 yeasts isolated from the Peking Union Medical College Hospital from January 2000 to December 20 01. Methods:Susceptibility testing was performed using a yeast disk diffusion method, Mueller Hinton agar supplemented with 2% glucose and 0.5 mg/L methylene blue was used. The diameter of inhibition zones of test and reference strain were read by BIOMIC System. Results:Strains of Candida albicans were the most frequent o rganism isolated which accounted for 54.7% of all the isolates. C. tropicalis, C. glabrata, C. parapsilosis, C. krusei and Cryptococcus neoformans acco unted for 18.7%, 12.1%, 4.7%, 3.7% and 3.7%, respectively. The susceptibility ra tes of C. albicans, C. tropicalis and C. parapsilosis to fluconazole wer e 92.5%—100%, their MIC 90 being in the range of <3—8 mg/L; while the MIC 90 of voriconazole against C. albicans, C. tropicalis, C. parasilosis and C. glabrata were 0.064 —1.5 mg/L. Conclusions:Fluconazole is active against C. albicans, C. trop icalis and C. parasilosis. Voriconazole is more active against C. albica ns, C. tropicalis,C. glabrata and C. parapsilosis than fluconazole.
出处
《中国抗感染化疗杂志》
2003年第5期277-279,共3页
Chinese Journal of Infection and Chemotherapy
