摘要
用3种杀虫剂及1种混剂对山东泰安花生蚜累代浸蘸处理,以选育抗药性品系。至16代,该虫对氧乐果未产生明显抗(?),对灭多威及氧乐果+灭多威(Al 2∶1)混剂抗性分别为19.7倍及11.4倍,而溴氰菊酯处理至12代抗性却高达113.9倍。上述后3个抗性品系对溴氰菊酯、灭多威及混剂存在高度或低(?)交互抗性,而对7种有机磷杀虫剂交互抗性不明显。经酶抑制剂生测、离体酶活性测定及同工酶谱分析,花生蚜对灭多威的抗性与水解代谢有关,对溴氰菊酯的抗性与水解和氧化代谢有关。
For selection of resistant strains, every generation of the peanut aphid native from Taian was treated by dipping with three insecticides individually and one mixed agent. Up to the 16th generation, the omethoate-selected strain had no resistance, but the methomyl alone and in combination with omethoate (AI 1:2) selected strains had 19.7 and 11.4 fold resistance respectively. The resistance of the deltamethrin-selected strain increased to 113.9 times at the 12th generation. The later three resistant strains showed cross-resistance at high or low level to deltamethrin, methomyl and the mixture and no significant cross-resistance to other seven organophosphorus insecticides tested. The bioassay with an enzyme inhibitor, determination of enzyme activity in vitro and analysis of esterase isozymes revealed that the resistance of the peanut aphid to methomyl was related to hydrolytic metabolism, and the resistance to deltamethrin related to hydrolytic and oxidative metabolism.
出处
《山东农业大学学报（自然科学版）》
CSCD
1992年第3期284-290,共7页
Journal of Shandong Agricultural University：Natural Science Edition
关键词
花生
苜蓿蚜
交互抗性
选育
peanut aphid
cross-resistances
resistant mechanism
carboxylesterase
esterase
isozyme
