摘要
目的 :探讨板蓝根抗内毒素作用机制。方法 :将具有抗内毒素作用的板蓝根氯仿提取部位 (F0 2 )及该部位中的F0 2 2 组份配制成 1%水溶液 (样品液 )。用电子显微镜观察经样品液作用前后内毒素结构形态的变化 ;用显色基质法检测样品液对内毒素的破坏作用 ;用样品对脂多糖致鼠巨噬细胞分泌炎性因子的抑制作用、对脂多糖刺激鼠组织moesimRNA分子表达影响及对脂多糖诱导鼠体内蛋白激酶MAPKP38、TNFα、IL 6和NO的抑制作用等探讨板蓝根抗内毒素的分子机制。结果 :经板蓝根样品液作用后 ,长链状且相互交叉成网状的内毒素呈不规则的短片状或颗粒状 ;0 5ml1%F0 2 液可使 2 0EU内毒素降解为 0 0 2EU ,破坏率为 99% ;1%F0 2 液和 1%F0 2 2液 2 0ng/ml对浓度为 5 0和 10 0ng/ml的脂多糖刺激巨噬细胞分泌TNFα、IL 6的抑制率分别为 84 4 % ,4 9 0 % ,77 6 % ,4 2 4 %和 78 6 % ,4 1 7% ,85 9% ,39 6 % ;1%F0 2 2 液对脂多糖刺激鼠肝、肾、脾组织moesimRNA分子表达的抑制率分别为 93 5 %、88 6 %和 87 8% ,平均抑制率为 90 1% ;1%F0 2 2 液对脂多糖刺激鼠体内蛋白激酶MAPKP38、TNFα、IL 6和NO的抑制率分别为 93 2 % ,96 6 % ,95 8%和 93 0 %。结论 :板蓝根氯仿提取部位 (F0 2 )及其F0 2
AIM: Studies on the Antiendotoxic mechanism of Radix Isatidis . METHOD: The content designed has finished on the basis of the facts identified by lots of experiments that the chloroform part (F02)from Radix Isatidis and F022(isolated from F02) to facture solution 1%(sample) . The endotoxin was shown to have change with the effect of solution on the electron mierocope ;Using coloration matrix as a method for the quantitation of endotoxin , pretreated by sample ; the delease of the inflammation factors stimulated by LPS was inhibited the influences on the molecular expression of moesin mRNA in mice tissues and MAPKp38,TNFα,IL6 and NO in mice for LPS 's effect of inhibition , studies on the Antiendotoxic mechanism of Radix isatidis . RESULT: With the sample of Radix isatidis effect to have a long chain and then to ture pieces with effect of solution ; It was found that 20EU of the endotoxins could be reduced from 20EU to 0.02EU by 0.5ml of 1% F02 solution and the reduction rate was 99% ; 1% F02 and 1% F022 (20 ng/ml) to 50 and 100 ng/ml concentration for the inflammation factors stimulated by LPS with excreled TNFα,IL6's restrain rate was 84.4%,49.0%,77.6%,42.4% and 78.6%,41.7%,85.9%,39.6% ;1% F022 stimulated mice's liver,kidney,spleen molecular expression of moesin mRNA stimulated by LPS was 93.5%,88.6% and 87.8%,restrain rate was 90.1% for average;1% F022 stimulated MAPKp38,TNFα,IL-6 and NO in mice stimulated by LPS was 93.2%,96.6%,95.8% and 93.0%. CONCLUSION: The chloroform part (F02) from Radix Isatidis and F022 not only the endotoxin was changed but also give voice to restrain the delease release with transition both in bodyside , restrain extend for film albumen and enzyme , clarify the mechanism on the Antiendotoxic Action of Radix Isatidis from level with molecule .
出处
《中国药科大学学报》
CAS
CSCD
北大核心
2003年第5期442-447,共6页
Journal of China Pharmaceutical University
基金
国家自然科学基金 (编号 3 9870 987)
卫生部科学研究基金资助项目 (编号 98 2 110 )~~
