摘要
以5年生厚荚相思(Acaciacrassicarpa)的萌芽茎段为外植体进行了组织培养的初步研究.结果表明:改良的MS+6 BA2.0mg/L+NAA0.2mg/L培养基能够较好地诱导芽的分化和增殖;适宜的继代增殖培养基为改良MS+6 BA1.5mg/L+NAA0.2mg/L;较为理想的生根培养基为1/2MS+IBA0.5mg/L,生根率达95.0%;生根苗的移栽基质以黄心土的移栽效果最好,移栽成活率达90.0%.
A tissue culture method is described for clonal multiplication of Acacia crassicarpa using nodal explants from the trees growing in the field. Results showed that the improved MS medium with 2.0 mg/L 6 BA and 0.2mg/L NAA was the optimum medium for induction and proliferation of bud clusters. The suitable culture medium for the generation continuing multiplication is the improved MS supplemented with 1.5mg/L 6 BA and NAA 0.2mg/L.The shoots rooted best on 1/2MS medium supplemented with 0.5 mg/L IBA. Plantlet survival after transfer to soil was 90.0%.
出处
《四川大学学报（自然科学版）》
CAS
CSCD
北大核心
2003年第5期982-985,共4页
Journal of Sichuan University(Natural Science Edition)
基金
广西科学基金(桂科基0342013)
广西林业科技项目(林科字[2000]第86号)
关键词
厚荚相思
快速繁殖
植株再生
Acacia crassicarpa,micropropagation, plant regeneration
