摘要
【目的】揭示广东汉族人群Y染色体小卫星DYF155S1基因座3'端多态性。【方法】采用Amp-FLP和荧光MVR-PCR方法扩增,PCR产物用ABI377电泳或中性聚丙烯酰胺凝胶电泳-银染方法分析。【结果】155例无关男性个体的4型核心序列MVR图谱第1条DNA片段大小均为133bp。155例共检出有24种等位基因,有7个等位基因仅出现1次,频率最高的为9号等位基因,DNA条带数为连续的13个,频率为0.1355;基因多样性(h)为0.9226。155人中有3人表现为连续DNA谱带中有1个DNA条带的缺失(nullrepeat),序列分析证明缺失的DNA条带位置对应于3型核心序列。【结论】揭示了DYF155S1位点3'端多态性及其等位基因频率,为群体遗传学研究及法医学应用提供了基础资料。
Objective] To reveal the diversity at 3′ end of DYF155S1 locus of Y specific minisatellite among Han population in Guangdong. Amp FLP method was used to amplify DYF155S1 fragments and retrieved DNA from agrose gel. The allele of type 4 core sequence from the 3′ end at DYF155S1 locus was amplified by using MVR PCR (minisatellite variant repeat by PCR). PCR products were analyzed by ABI377 sequencer or polyacrylmide gel electrophoresis followed by silver staining. 155 unrelated males among Chinese Han population in Guangdong were genotyped. The size of the first DNA fragment of type 4 MVR mapping of all individuals was 133 bp. Among 155 samples 24 alleles were found,7 out of them were observed once only. The most frequent allele was No. 9, at frequency 0.135 5. The gene diversity (h) was 0.922 6. Out of 155 samples, 3 samples had one band lost (null repeat) among the successive bands. It was demonstrated by sequencing that the lost band was type 3 repeats. [ Conclution] We reveal the minisatellite variant repeat of 3′ end diversity of DYF155S1 locus by fluorescent detection and provide the frequency data of DYF155S1 locus in Han population in Guangdong for studying the population genetics and for forensic practices.
出处
《中山大学学报（医学科学版）》
CAS
CSCD
北大核心
2003年第5期435-438,共4页
Journal of Sun Yat-Sen University:Medical Sciences
基金
广东省自然科学基金资助项目(97-128)
中山医科大学"211工程"重点学科建设课题基金资助项目(4209008)
