摘要
目的 :构建人血管内皮生长因子 ( VEGF) c DNA逆转录病毒表达质粒。方法 :采用 PCR方法从 pc DNA- VEGF165质粒中扩增 VEGF165基因 ,在序列两端分别引入 Eco R 和 Bam H 限制性内切酶识别位点 ,并克隆到逆转录病毒表达载体 p LXSN质粒中。结果 :酶切鉴定、PCR扩增以及DNA序列分析表明已经将 VEGF165基因全长序列克隆到逆转录病毒表达载体 p LXSN质粒中 ,获得了 p LXSN- VEGF165重组质粒。结论 :成功地构建了人 VEGF c DNA逆转录病毒表达质粒 ,为进一步临床应用 VEGF c
Objective:To construct retroviral vector expression plasmid with human VEGF 165 gene. Methods:Human VEGF 165 gene was amplified from pcDNA VEGF 165 plasmid by PCR and the direction of restriction endonucleases sites (BamHⅠand EcoRⅠ) on the both ends of VEGF 165 cDNA was adjusted, then they were cloned into pLXSN retroviral expression vector. Results: The retroviral vector expression plasmid containing whole VEGF 165 cDNA sequence was constructed, its sequence was corrected by DNA direct sequencing and named pLXSN VEGF 165 . Conclusion:The retroviral vector expression plasmid with human VEGF 165 gene is constructed successfully, lay the foundation for studying VEGF gene therapy. [
出处
《吉林大学学报（医学版）》
CAS
CSCD
北大核心
2003年第5期627-629,共3页
Journal of Jilin University：Medicine Edition
基金
吉林省卫生厅资助课题 (2 0 0 1科基字 193号 )
